Sequences from confirmation amplicon


Confirmation sequences for line SALK_028169c and hit At3g14020 are shown.
Line ID SALK_028169c
Locus At3g14020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4643650
Primers used for PCR BN29 - LBb3, show primer details
   
Sequence Name 49-K107479-SALK_028169C-BN29
GenBank Accession LN485473 [GenBank]
Sequencing primer gene specific primer, BN29: 5'-TTTGATTCTTGATGCTTCTTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K107479-SALK_028169C-BN29
TCTACTCTCTTAGTGCATGGAGGTGCCGCGACTCGTGAAGATATGGCTGCATAGTTTCAT
ACAAAAAATTGGTCTTGAGTCAAGACTCTTGACATAATAACTGAAACTTTCTGCATATAA
TTCTATTTATCAGAGTGGTGCAATATAGATAAACAAACATACTTTGCGGACTTTGATGAG
CTTGTTCTGAGCTTCAAGCTTTGCACGGCGCTCTCTGCGGCGGAGAATGGCTTGGTACTG
TTTTGCATTGACGAAAATTGGTTCGTTCTCTGGAATGTTGTGAGGCAAAGGCATTCTAGA
CGGAACCAACCCTCTGATTTGAGGATGCTGCACCTGCAAAGATTTCCATAACAAATACTT
TCATAAAGATACAAGAAACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCGAATCNGGCAAAA
   
Sequence Name 65-K107480-SALK_028169C-LBb3
GenBank Accession LN485865 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K107480-SALK_028169C-LBb3
GGGCAACAGCGTGGACCGCTTGCTGCACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAG
CTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCAA
TGTGTTATTAAGTTGTCTAAGCGTCAATGTTTCTTGTATCTTTATGAAAGTATTTGTTAT
GGAAATCTTTGCAGGTGCAGCATCCTCAAATCAGAGGGTTGGTTCCGTCTAGAATGCCTT
TGCCTCACAACATTCCAGAGAACGAACCAATTTTCGTCAATGCAAAACAGTACCAAGCCA
TTCTCCGCCGCAGAGAGCGCCGTGCAAAGCTTGAAGCTCAGAACAAGCTCATCAAAGTCC
GCAAAGTATGTTTGTTTATCTATATTGCACCACTCTGATAAATAGAATTATATGCAGAAA
GTTTCAGTTATTATGTCAAGAGTCTTGACTCAAGACCAATTTTTTGTATGAAACTATGCA
GCCATATCTTCACGAGTCGCGGCACCTCCATGCACTAAAGAGAGTTAGAGGCTCTGGTGG
ACGTTTCCTCAACACAAAGAAGCATCAGGAATCAA
Primers used for PCR BN30 - LBb3, show primer details
   
Sequence Name 82-K118526-SALK_028169C-BN30
GenBank Accession LN486249 [GenBank]
Sequencing primer gene specific primer, BN30: 5'-AGGTCCACCACTTTTTAACCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K118526-SALK_028169C-BN30
ATTTAATTTTAGCTTATCTATGGTTTCTTGTTTTGGTGCAGGTTACAAAGAAAACCCGGA
AAATCCTATTGGAAGTCATTCGATATCAAAGGTGTCTCAAGATTCAGTGGTTCTTCCTAT
TGAGGTTAATTATTAGACTATCATCTTTGACCTTATTATAAACCTTGTAGTAGTTCAAGA
TGAGACTGAGTTTAAAGCTGAATACTATCTTAATGATGTCTATATATGCTCACAGGCGGC
TTCTTGGCCTTTACACGGCAATGTAACGCCACATTTCAATGGTTTCTTGTCTTTTCCTTA
TGCATCACAACACACGGTAATACACAATTGTAACATTTCAAGATAATTTTAATAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
C
   
Sequence Name 72-K118525-SALK_028169C-R210
GenBank Accession LN486067 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K118525-SALK_028169C-R210
ATTAAAATTATCTTGAAATGTTACAATTGTGTATTACCGTGNTGTTGTGATGCATAAGGA
AAAGACAAGAAACCATTGAAATGTGGCGTTACATTGCCGTGTAAAGGCCAAGAAGCCGCC
TGTGAGCATATATAGACATCATTAAGATAGTATTCAGCTTTAAACTCAGTCTCATCTTGA
ACTACTACAAGGTTTATAATAAGGTCAAAGATGATAGTCTAATAATTAACCTCAATAGGA
AGAACCACTGAATCTTGAGACACCTTTGATATCGAATGACTTCCAATAGGATTTTCCGGG
TTTTCTTTGTAACCTGCACCAAAACAAGAAACCATAGATAAGCTAAAATTAAATGCAGTT
GACGCAGCAATGTGAACTCTTACTCAGGCGAAAAAGGTTAAAAATGGGTGGACCT

Last Updated on 06.12.2018 16:11