Sequences from confirmation amplicon


Confirmation sequences for line SALK_028578c and hit At4g01950 are shown.
Line ID SALK_028578c
Locus At4g01950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:845693
Primers used for PCR 13jg - LBb3, show primer details
   
Sequence Name 22-K113259-SALK_028578C-13jg
GenBank Accession LN484918 [GenBank]
Sequencing primer gene specific primer, 13jg: 5'-TTTTGCCAGGTCAGTCTCTTTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113259-SALK_028578C-13jg
TTTCGATTAACAGTTTTAATTATATTTGGTAAGAGATTTTTTGGAAATACTAAACGAGAA
TGTTTTAACTACTAAATATTGAAGCAGCCAAAAAAAGAAACTACTCCATATATGTTGATA
TCTATATTATGACTTGTTACAGTTTTTATATATATGATGACCGATAATTAGTAAAAAGCA
ACATAGCATATTAAATTGTGATAGTAGTGACGGTGATGAAACACGGACAACTTGCTTGAT
TGCGAATATGAAATATTAACAACATAACGTCAAAAATATTAACAACATATATAAAAAGGG
AAAACAAAAGGAAAGCCCTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAG
AGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTG
GTTCCGAAATCGGCAAAA
   
Sequence Name 05-K113258w-SALK_028578C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K113258w-SALK_028578C-R210
CTTTTATAATGTTGTAATATTTTTGACGTTATGTTGTTAATATTTCATATTCGCAATCAA
GCAAGTTGTCCGTGTTTCATCACCGTCACTACTATCACAATTTAATATGCTATGTTGCTT
TTTACTAATTATCGGTCATCATATATATAAAAACTGTAACAAGTCATAATATAGATATCA
ACATATATGGAGTAGTTTCTTTTTTTGGCTGCTTCAATATTTAGTAGTTAAAACATTCTC
GTTTAGTATTTCCAAAAAATCTCTTACCAAATATAATTAAAAACTGTATAATCTGTAAAC
GTAAACGCAAACAAAATAATGATAAAAGAGACTGACCTGGCAAA

Last Updated on 06.12.2018 16:11