Sequences from confirmation amplicon


Confirmation sequences for line SALK_029828c and hit At1g23030 are shown.
Line ID SALK_029828c
Locus At1g23030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8156796
Primers used for PCR 08ii - LBb3, show primer details
   
Sequence Name 35-K112623-SALK_029828C-08ii
GenBank Accession LN485220 [GenBank]
Sequencing primer gene specific primer, 08ii: 5'-TCTCAAGCTTCCATGTAACACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112623-SALK_029828C-08ii
ATCCTTTATCAACAAATTCAAATCTTTCAGCTAAATCGGAGATAACCGTATTGTAACCAA
TGGTCTAATTACATTTAACCGGTAATTCTAATGAATCTGGGAATTTTCATGAGATTGAAG
AACAACAAATCGTGATTTGATTTTTTTTAAGGAGTTAGTTAGATTTTTTTGCTTACAGAG
GAATCGCGAGCTTGGAATCGAGCTGTGGAGAGAAGACGCTTCGCGGCTTGAAGTCCGACC
ACGAGATCAGACCACCAATCATTCTCCGACGACGACGAAGCGGCGGAATCGATAGGAGTT
GAGTCTCTAATCTCCTCTAACAAATGCGTTAAGAGACAAACTCGTCGAGTGAGATCTGCG
CAATCCTTCTTGAACATTCCGGTGTTGAGTGGAATCTCGACGATNAGAGCTTAGACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGAATCAGGCAAAA
   
Sequence Name 11-K112622-SALK_029828C-R210
GenBank Accession LN484585 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112622-SALK_029828C-R210
GTCATTTGTCTAGCTCTAATCGTCGAGATTCCACTCACACCGGAATGTTCAAGAAGGATT
GCGCAGATCTCACTCGACGAGTTTGTCTCTTAACGCATTTGTTAGAGGAGATTAGAGACT
CAACTCCTATCGATTCCGCCGCTTCGTCGTCGTCGGAGAATGATTGGTGGTCTGATCTCG
TGGTCGGACTTCAAGCCGCGAAGCGTCTTCTCTCCACAGCTCGATTCCAAGCTCGCGATT
CCTCTGTAAGCAAAAAAATCTAACTAACTCCTTAAAAAAAATCAAATCACGATTTGTTGT
TCTTCAATCTCATGAAAATTCCCAGATTCATTAGAATTACCGGTTAAATGTAATTAGACC
ATTGGTTACAATACGGTTATCTCCGATTTAGCTGAAAGATTTTGAATTTTGTTGATTAAA
GGATGGTGCTGCGAAGAGAATCTCATTTCAGTTCCAATGTGTTACATGGAAGCTTGAGA

Last Updated on 06.12.2018 16:11