Sequences from confirmation amplicon


Confirmation sequences for line SALK_030651c and hit At5g19380 are shown.
Line ID SALK_030651c
Locus At5g19380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6526842
Primers used for PCR 18ma - LBb3, show primer details
   
Sequence Name 83-K114279-SALK_030651C-18ma
GenBank Accession LN486260 [GenBank]
Sequencing primer gene specific primer, 18ma: 5'-TGCTTTCATGGAAACCTACTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K114279-SALK_030651C-18ma
CATCTATGTGATATCTTATAAGTTCAACAATGCTGTACCAATCCTATAAACAACCAAACT
TTTTTTGGTCATAAATTTTGGTGGAATGGCATAGGAGTTAGCATCATTTATAAGCAGAAG
AGAAACATTTGTAATATACAGAACCTTGTTTGGCTTTGTCGATGCAGTGCCCATAGCGGT
GTACTGTTTCACGCTTCCCCTACCATACCTTGGGGTGGCATCTACACTACCAAGGGGGTT
TGTTGCAGGCACAATCATCCTTGTAGTCGGCATGCTTCTATATGCTTGGACACCATCTAC
TAACACTTCCGATTCAATTATCCCCTCACCCCCATCCACATAGCGGCAGGAAAATTGGTT
TTTGTAACATTATTTGGTTTCCTTCTCTCTTCTCATCTTAGCATATGCTCTTTTTTTGTC
AAATAAGAAATTTTTTTATGCATTCTTTACAAAAGAATGGTTAGTTTTCAGTTTTGCTTT
TCAAAAACTATGCAGAGGATAATGTACTTTGTAATGATGTATATCAAATTGACGCTNAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 69-K114278-SALK_030651C-R210
GenBank Accession LN485984 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K114278-SALK_030651C-R210
ATCATTACAAAGTACATTATCCTCTGCATAGTTTTTGAAAAGCAAAACTGAAAACTAACC
ATTCTTTTGTAAAGAATGCATAAAAAAATTTCTTATTTGACAAAAAAAGAGCATATGCTA
AGATGAGAAGAGAGAAGGAAACCAAATAATGTTACAAAAACCAATTTTCCTGCCGCTATG
TGGATGGGGGTGAGGGGATAATTGAATCGGAAGTGTTANTANATGGTGTCCAAGCATATA
GAAGCATGCCGACTACAAGGATGATTGTGCCTGCAACAAACCCCCTTGGTAGTGTAGATG
CCACCCCAAGGTATGGTAGGGGAAGCGTGAAACAGTACACCGCTNTGGGCACTGCATCGA
CAAAGCCAAACAAGGTTCTGTATATNACAAATGTTTCTCTTCTGCTTATAAATGATGCTA
ACTCCTATGCCATTCCACCAAAATTTATGACCAAAAAAAGTTTGGTTGTTTATAGGATTG
GTACAGCATTGTTGACTTATAAGATATCACATAGATGATGATGGTATATCATTGGTAATT
ACTAATTCGCTGAAGAAGTAGGTTTCCATGAAAGC

Last Updated on 06.12.2018 16:11