Sequences from confirmation amplicon


Confirmation sequences for line SALK_031569 and hit At5g66770 are shown.
Line ID SALK_031569
Locus At5g66770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26662194
Primers used for PCR 20ea - LBb3, show primer details
   
Sequence Name 60-K110715-SALK_031569C-20ea
GenBank Accession LN485739 [GenBank]
Sequencing primer gene specific primer, 20ea: 5'-AGAATTCAAAACCTACCCCTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K110715-SALK_031569C-20ea
GAAACAACACTCTGACCCGGAGTATATTAACAATTGAATATTTGTCTAACCAAAAATAAC
ATTGAAAACAAAACAAAACTAAGAATACCGGATCGGTTTGGTTTGGTTATCGCCAGGAAG
AAAGAGTGAGGAGAGGTAAATCGTTCCAGGCCAAAGAGATGAAGCCAGGCTTAGATTCAA
CAATTGAATACAAATTGCTGTAATTGTAATTCCACAATAGAATCTTCGCTTGGCTCACTG
CGTAATTACTCAGCTTAACCGATTCAAAACCGGCATTCTCCATTAATACCCGCCATTGCT
CTTTCTCTTCCATTCTTTCTCTATGAATTCCGGTTTTCTCCGGTCCAATCAAACCCGAGA
TTCTCCGGCCGAACAACTCTCGCTCAACTCCAGGATATATTGTGGTGTAAACAAATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCAAA
AATGGGCAAAA
   
Sequence Name 44-K110714-SALK_031569C-R210
GenBank Accession LN485372 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K110714-SALK_031569C-R210
GTCATTTGTTTACCCACAATATATCCTGGAGTTGAGCGAGAGTTGTTCGGCCGGAGAATC
TCGGGTTTGATTGGACCGGAGAAAACCGGAATTCATAGAGAAAGAATGGAAGAGAAAGAG
CAATGGCGGGTATTAATGGAGAATGCCGGTTTTGAATCGGTTAAGCTGAGTAATTACGCA
GTGAGCCAAGCGAAGATTCTATTGTGGAATTACAATTACAGCAATTTGTATTCAATTGTT
GAATCTAAGCCTGGCTTCATCTCTTTGGCCTGGAACGATTTACCTCTCCTCACTCTTTCT
TCCTGGCGATAACCAAACCAAACCGATCCGGTATTCTTAGTTTTGTTTTGTTTTCAATGT
TATTTTTGGTTAGACAAATATTCAATTGTTAATATACTCCGTGGTCAGAGTGTTTTGTTT
TTCTTTTAGTTCGAACGTTGAATTAATTCAGGGGTAGGTTTTGAATTC

Last Updated on 06.12.2018 16:11