Sequences from confirmation amplicon


Confirmation sequences for line SALK_032042 and hit At1g17560 are shown.
Line ID SALK_032042
Locus At1g17560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6037991
Primers used for PCR 23kn - LBb3, show primer details
   
Sequence Name 68-K113914-SALK_032042C-23kn
GenBank Accession LN485957 [GenBank]
Sequencing primer gene specific primer, 23kn: 5'-CATTCACTCTCTGCGACACTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113914-SALK_032042C-23kn
ATATCGTCATGGACTTCGCTTCGGTTCATGAAATGCAGACTCAAGATTAAAACTTTTTTG
TTCTTGTTGTGAATGTCACTAATTGTTGGTGATGTCGTTATATGATTCATCGACAATATG
AGTAGGACGTTCATTGCTTGGAGGTCTTTGCAATGCTTTCTCTGGTTTGATGAATTCCTC
CTCCAATGGAATGATGAATGGAAGCATCCTCTCTCAGGTCAAGCCTCTTTTCGCAAATAT
ACGTCCTTGATGAAACTTTCTTCATATGCATTCGTGGGGTATAACCTGTGAATTGTGTGT
CCTGATGATTTCCAATTCACAGCAACAACATAGGACATTCATTCAAATGGGGACGATTCT
CAAATGCGTGGATAACTCGTGTGCTAAAGAGGTGATGTGCATTCAATCCCTGAGAGGTAA
GAAAGGAGCAACAAAATAACACATTAATAACACATTGAACAAATTGACGCTTAGACAACT
TAATAACATTAATATTGTGAAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTG
   
Sequence Name 57-K113913-SALK_032042C-R210
GenBank Accession LN485666 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K113913-SALK_032042C-R210
TTATTTTGTTGCTCCTTTCTTACCTCTCAGGGATTGAATGCACATCACCTCTTTAGCACA
CGAGTTATCCACGCATTTGAGAATCGTCCCCATTTGAATGAATGTCCTATGTTGTTGCTG
TGAATTGGAAATCATCAGGACACACAATTCACAGGTTATACCCCACGAATGCATATGAAG
AAAGTTTCATCAAGGACGTATATTTGCGAAAAGAGGCTTGACCTGAGAGAGGATGCTTCC
ATTCATCATTCCATTGGAGGAGGAATTCATCAAACCAGAGAAAGCATTGCAAAGACCTCC
AAGCAATGAACGTCCTACTCATATTGTCGATGAATCATATAACGACATCACCAACAATTA
GTGACATTCACAACAAGAACAAAAAAGTTTTAATCTTGAGTCTGCATTTCATGAACCAAA
GCAAAGTCCATGACATATCACAAACCCAAAAACTCAAAGCTCATTTCGAAACTTATACTA
AATCAATGTTGCAAAAAATCTAATTTTTAAAGAACCAAACTTTATCGAATATAATAATAT
TAAGAGATGAAAATGTACCTTTGGAGAGCTTGGAAGCTAAAGCTGTCGCCATTTCTGCAA
TTCAAAAGTTTCGATCTTTAGTGTC

Last Updated on 06.12.2018 16:11