Sequences from confirmation amplicon


Confirmation sequences for line SALK_032139 and hit At1g14290 are shown.
Line ID SALK_032139
Locus At1g14290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4881763
Primers used for PCR 07bh - LBb3, show primer details
   
Sequence Name 20-K108069-SALK_032139C-07bh
GenBank Accession LN484839 [GenBank]
Sequencing primer gene specific primer, 07bh: 5'-ACATTAAAATTGAAACCAGTTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K108069-SALK_032139C-07bh
TTAGCCCGTGGAATTCCGAATAGTTGGGTCCCCCTGAAAAATCACGTTAGATCTCTTCAT
CATCTCCTCCTCTGCATTTTCTTCTCCTATTCTTCTCGACGACGAAAGAAAACACCGAAG
CAACGAGGAAGAAGAAGATTATAATCTAATCTCTCCTTCGTGTAGTTTAGCGAAGAGTGA
TGCGATTTTCTTCTGGCGGTGTTTGATTTCGTATTCTCGAGTTGTTGTGGGGGAGAGATT
GATTAGAATTAGAGATGATGAGTTTCGTGATTTCAGGATATATTGTGGTGTAAACAAATT
GACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
CGAGATCGGCAAAA
   
Sequence Name 31-K108070-SALK_032139C-R210
GenBank Accession LN485141 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K108070-SALK_032139C-R210
GCGTCATTTGTTTACCCCAATATATCCTGAAATCACGAAACTCATCATCTCTAATTCTAA
TCAATCTCTCCCCCACAACAACTCGAGAATACGAAATCAAACACCGCCAGAAGAAAATCG
CATCACTCTTCGCTAAACTACACGAAGGAGAGATTAGATTATAATCTTCTTCTTCCTCGT
TGCTTCGGTGTTTTCTTTCGTCGTCGAGAAGAATAGGAGAAGAAAATGCAGAGGAGGAGA
TGATGAAGAGATCTAACGTGATTTTTCAGGTGGGACCCAACTATTCGGAATTTTCCACGT
GGCTTTAAACGAATGGTCAAGGCGCCAACTGGTTTCAATTTTAATG

Last Updated on 06.12.2018 16:11