Sequences from confirmation amplicon


Confirmation sequences for line SALK_032652c and hit At4g39610 are shown.
Line ID SALK_032652c
Locus At4g39610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18394010
Primers used for PCR 33dr - LBb3, show primer details
   
Sequence Name 43-K110394-SALK_032652C-33dr
GenBank Accession LN485351 [GenBank]
Sequencing primer gene specific primer, 33dr: 5'-TTCATGAATTCACATCTTTTCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K110394-SALK_032652C-33dr
CTCTCACATGGTAATTTTAAATATTCAAAAATATTGATGGTTTTCAATGTTTACTAATTG
TATGTGAAGAAAAGGAAGAAAGGGTTTGGGATAAAATTGTGATCAAAGAAATCAAGATTG
ATGTTGGTGGAAGTAATTCAAGACGTGCAGGTCAAGGAAATGCATCAGATCCAGGCACCA
AGATCTAGCGACAGTTTACCAATGATTATTATTACTAACTCGGACAATTTAGAAGAGTTC
CGTAGATTCAAATTCATTCCATTTTTTAAACTAAAAGATATATGTCTATAAGTTTTGTCA
ATTAAACCTCCTCCTATCCAATGTCATCACATACATTTCCTTTCAGGACGAAGAAGTGTG
TATATACACACACTTACAATTCAATCTTTTTATAAAGATCGAGAGATTAACAACGAAAGA
ACAACAAAAAATTCTTACAATTATCCTAAACAATAGTATATAACGTCGACGACCACCGCC
GTTTCCGGTCATTTACATACACGTTACATTAATTTGAACCATAACAAAATAAGTCAAACT
CTCACAAAGAAAAAGCTAAGTTCGGGTCCATTATTCCCTTCAGGACTCAACATATAAAAA
GTCTCTGAATCTTTAGACCCAATAACTCTCTCAAAATAAGCTCTCATATACGCCATTTCG
CCGTCGGGTCCTTCCGACTCCGAGTTTCCCGGCAAAACTCCGGCGCCCATAGACACCGGA
CGTAACAACTCCATCANGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTG
   
Sequence Name 35-K110393-SALK_032652C-R210
GenBank Accession LN485218 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K110393-SALK_032652C-R210
GCGTCATTTGTTTACCCACAATATATCCTGATGGAGTTGTTACGTCCGGTGTCTATGGGC
GCCGGAGTTTTGCCGGGAAACTCGGAGTCGGAAGGACCCGACGGCGAAATGGCGTATATG
AGAGCTTATTTTGAGAGAGTTATTGGGTCTAAAGATTCAGAGACTTTTTATATGTTGAGT
CCTGAAGGGAATAATGGACCCGAACTTAGCTTTTTCTTTGTGAGAGTTTGACTTATTTTG
TTATGGTTCAAATTAATGTAACGTGTATGTAAATGACCGGAAACGGCGGTGGTCGTCGAC
GTTATATACTATTGTTTAGGATAATTGTAAGAATTTTTTGTTGTTCTTTCGTTGTTAATC
TCTCGATCTTTATAAAAAGATTGAATTGTAAGTGTGTGTATATACACACTTCTTCGTCCT
GAAAGGAAATGTATGTGATGACATTGGATAGGAGGAGGTTTAATTGACAAAACTTATAGA
CATATATCTTTTAGTTTAAAAAATGGAATGAATTTGAATCTACGGAACTCTTCTAAATTG
TCCGAGTTAGTAATAATAATCATTGGTAAACTGTCGCTAGATCTTGGTGCCTGGATCTGA
TGCATTTCCTTGACCTGCACGTCTTGAATTACTTCCACCAACATCAATCTTGATTTCTTT
GATCACAATTTTATCCCAAACCCTTTCTTCCTTTTCTTCACATACAATTAGTAAACATTG
AAAACCATCAATATTTTTGAATATTTAAAATTACCATGTTGAGAGTCATACGGATGCTTT
CTAATGATCGTCCGTGCAGAAAAGATGTGAATTCATGAAAA

Last Updated on 06.12.2018 16:11