Sequences from confirmation amplicon


Confirmation sequences for line SALK_032851c and hit At3g21465 are shown.
Line ID SALK_032851c
Locus At3g21465 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7562904
Primers used for PCR 28jr - LBb3, show primer details
   
Sequence Name 54-K113814-SALK_032851C-R210
GenBank Accession LN485607 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113814-SALK_032851C-R210
TTTGTTACACCACAATATATCCTGATGAGAAGAAGTCAGCACTTGAGGCTGAATTCAAGA
AGCTGGTAAACGAGTGGTCTGTTGACATTATCAAGCACCAAAATGAACAAGATAAGAAGG
TAGAACTCACTCCTCTTTATGTTTACCTAAAACCAAAAAAGCTCGTAACCTAAACCATGT
CTCCTTAAAAAAACAGGACGTAGCTGCATCACTGAAATCTGACATCCCTGCAATGGTCAA
TGCTTTGGTAAACTCTGGTTTGAGAGTAAGAGTGGATTTAAATGAGTTAAACAAGAATGA
AGCTCTTCTTAGCTGATGATTGGATTGATCCATTTTACCGCAACTAGAAGATCAGTTCAA
GACAGAAACAAAACATTTCTGTTCAGTTCCGTGCATGCTACATTAGCTGTTTCCTCGTTC
TTTTAGGGTTTGTCTTTAGGACACTCAAATAACCAAATTTGAAAAGAAGAAAAAAACAAG
AATTTTGATTCTCAAAGAGACCTTTGTACTTTGATCAACGAAGCAAACTCTCATTTCCTT
GAAAGTGTACTTTTTTATAAGTCAATATTATTACAAGACTAGCGAAGAGTGTTTCTTTTT
TTGGTCACAGTGATTGATGAATTTGGAGGAA
   
Sequence Name 67-K113815-SALK_032851C-28jr
GenBank Accession LN485932 [GenBank]
Sequencing primer gene specific primer, 28jr: 5'-CCTAACGATAAATGTTCCTCCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113815-SALK_032851C-28jr
AACACTCTTCGCTAGTCTTGTAATAATATTGACTTATAAAAAAGTACACTTTCAAGGAAA
TGAGAGTTTGCTTCGTTGATCAAAGTACAAAGGTCTCTTTGAGAATCAAAATTCTTGTTT
TTTTCTTCTTTTCAAATTTGGTTATTTGAGTGTCCTAAAGACAAACCCTAAAAGAACGAG
GAAACAGCTAATGTAGCATGCACGGAACTGAACAGAAATGTTTTGTTTCTGTCTTGAACT
GATCTTCTAGTTGCGGTAAAATGGATCAATCCAATCATCAGCTAAGAAGAGCTTCATTCT
TGTTTAACTCATTTAAATCCACTCTTACTCTCAAACCAGAGTTTACCAAAGCATTGACCA
TTGCAGGGATGTCAGATTTCAGTGATGCAGCTACGTCCTGTTTTTTTAAGGAGACATGGT
TTAGGTTACGAGCTTTTTTGGTTTTAGGTAAACATAAAGAGGAGTGAGTTCTACCTTCTT
ATCTTGTTCATTTTGGTGCTTGATAATGTCAACAGACCACTCGTTTACCAGCTTCTTGAA
TTCAGCCTCAAGTGCTGACTTCTTCTCATCAGGATATATTGTGGTGTAAACAAATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11