Sequences from confirmation amplicon


Confirmation sequences for line SALK_032878c and hit At2g17650 are shown.
Line ID SALK_032878c
Locus At2g17650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7671025
Primers used for PCR CF64 - R204, show primer details
   
Sequence Name 51-K107203-SALK_032878C-CF64
GenBank Accession LN485526 [GenBank]
Sequencing primer gene specific primer, CF64: 5'-ACTGAGAGATCAACACAAGCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107203-SALK_032878C-CF64
ATGAGCAATCTCAAGTAACTGATGATCAACAAAGAGGATTTTGGCCTCGGAGTGTGCTAA
CAAAACCGACAATGTGGAAGGATCAAGTCGAGTATTAAGCGGACAAAGAATCAAACCAGC
CATAGGAACAGCGAAATGAAGCTCATGCATAGCTGGAACATTCGGAGCTAAAGCTGCAAC
CTATAAAAGTTTCCTCTTTTATCACTAAACCTAAATCGAGAACCCTAATCAGAGCAGAAA
ATTGAACAATTTAAAGAGACTAACCACATCGCCACGAGAGATTCCGAGATTGGTAAGAGC
AGAGGCAAGACGGAGACAACGTTGATAAGTTTGGAACCAAGTGTGTTTAACGGAACCAAA
CACAAGAGAGGTTCTGTCTCTGTAAACCTTAGCGGATCGCTCCAAGAACGTGATTGGAGA
TAAAGGAGAGAAATTTGCAGGGGAACGGAGAAGACCCTCTATAGTTCTCCATGATTCCGG
CTCGGAATCGTCGGGAAATCCGCCGGAATTTGAGGTTGAGGAGAAGGGAGAAGAAGTTAA
CCACAGTCTCTGGAAACGTGGGTTGAAGATTCTGAATGCTCTTTTGGTTAACAAGAATCT
CATGGTTAACACTTTGATTTGGCGCTTAGACAAATTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
   
Sequence Name 43-K107202-SALK_032878C-R210
GenBank Accession LN485346 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K107202-SALK_032878C-R210
CGTCATTTGTCTAGCGCCAATCAAAGTGTTAACCATGAGATTCTTGTTAACCAAAAGAGC
ATTCAGAATCTTCAACCCACGTTTCCAGAGACTGTGGTTAACTTCTTCTCCCTTCTCCTC
AACCTCAAATTCCGGCGGATTTCCCGACGATTCCGAGCCGGAATCATGGAGAACTATAGA
GGGTCTTCTCCGTTCCCCTGCAAATTTCTCTCCTTTATCTCCAATCACGTTCTTGGAGCG
ATCCGCTAAGGTTTACAGAGACAGAACCTCTCTTGTGTTTGGTTCCGTTAAACACACTTG
GGTTCCAAACTTATCAACGTTNGTCTCCGTNCTTGCCTCTGNCTCTTACCAATCTCNGGA
ATCTCTCGTGGGCGATNNGTGGTTAGTCTCTTTAAATTGGTTCAANTTTTCTGGCTCTGA
TTNNAGGGTTCTCGATTTAGGGTTTAGTGGATAANAAGNAAGGAAACTTTTATAGGGTTG
GCAGNNCTTTAGCTCCGAAATGGTTCCNNAGCTATGCATNNGAGCTTCNNATTTCGCTGT
NNTCCTATGGCTGNNGTTTGATTCTTTGTCCGNCTTAATANNCTCGACTTGATCCNTTCC
ACATTGGGTCGGTTTTGNTTAGCACANNCTCCGAGGCCAAAANNTCCTCTTTGTTNGATC
ATCAGNNTTACTTGAGATTGGGCTCATGGAGNNCTCTTGATCTTCNNTTGCTAAATCAGA
AATAAAACTAGAAAAAGTCNTGAAGCTTGGTGTTGATTCTCCTCTCA

Last Updated on 06.12.2018 16:11