Sequences from confirmation amplicon


Confirmation sequences for line SALK_033186c and hit At1g14240 are shown.
Line ID SALK_033186c
Locus At1g14240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4865184
Primers used for PCR 27dh - LBb3, show primer details
   
Sequence Name 74-K109878-SALK_033186C-27dh
GenBank Accession LN486100 [GenBank]
Sequencing primer gene specific primer, 27dh: 5'-TTTCCCAACTTATCCAAAATGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K109878-SALK_033186C-27dh
TAACATTAGCCCATTCGTCTCGAAAATAAAACCTGAAGATCTAAGAACTCTTCTTGTAAC
TTCAAGAATCTGTTCTTGAACAGGCACTTCAAGTAATCTCATCCCAGCAGTCGCCATTAA
CCTAATGTCACTCCTCCTGAACATTCTCTTGGGAATCCTCTGCTTGGCGAATTCCACAAG
CTTCGTCACTGACACGCTTGCTCCCTCCGGATTATCAGCATACGAAGACAAACCAGGAGT
CAACTTCAGATTAGCGTAATGTTTCTCTCCGAAATCGAAAACGGGTTTCCCGGACTCAAA
CCAGTACCCGAACACATGAACCCGGGTCCCTGAACTCCCTGCGTCGATAAGGACGCTGTA
CCGGAGCTTACATCTCCGACTAAGCAAACTTCCGGAAGATATAACAGAATTTGAGTTGAA
AACATATAATAATAACCCTAACGTGATAGTCACGGAAACGACAACTAGCAGGATCAATGA
TTTCGACTTGGCCTTCGTGACTGTGTAGGGCAATGACTGGTGCTTAGGTAGGATCTGAAC
TTAA
   
Sequence Name 72-K109877-SALK_033186C-R210
GenBank Accession LN486051 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K109877-SALK_033186C-R210
TCAGCGTCATATGTTAAGTTCAGATCCTACCTAAGCACCAGTCATTGCCCTACACAGTCT
CGAAGGCCAAGTCGAAATCATTGATCCTGCTAGTTGTCGTTTCCGTGACTATCACGTTAG
GGTTATTATTATATGTTTTCAACTCAAATTCTGTTATATCTTCCGGAAGTTTGCTTAGTC
GGAGATGTAAGCTCCGGTACAGCGTCCTTATCGACGCAGGGAGTTCAGGGACCCGGGTTC
ATGTGTTCGGGTACTGGTTTGAGTCCGGGAAACCCGTTTTCGATTTCGGAGAGAAACATT
ACGCTAATCTGAAGTTGACTCCTGGTTTGTCTTCGTATGCTGATAATCCGGAGGGAGCAA
GCGTGTCAGTGACGAAGCTTGTGGAATTCGCCAAGCAGAGGATTCCCAAGAGAATGTTCA
GGAGGAGTGACATTTGGGTAAT

Last Updated on 06.12.2018 16:11