Sequences from confirmation amplicon


Confirmation sequences for line SALK_033970c and hit At1g14350 are shown.
Line ID SALK_033970c
Locus At1g14350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4910336
Primers used for PCR 53us - LBb3, show primer details
   
Sequence Name 06-K117668-SALK_033970C-R210
GenBank Accession LN484441 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117668-SALK_033970C-R210
TCATTTGTTTCACCACAATATATCCTGATGAGAATATGCCCATCTCAGGCGAAGAAAAGT
TCAGCTCGCCTATTCAGGTCACGCCATTGTTCAGATCTTTGGCAGACGGTATACCAAGTC
CACAGTTCTCCGAAAGTGTAAGCTTCGCACCTTCTTTGTTCATCATACAATACTTAGGCT
TTGAAAAAACAATGAGTCTTAAAAGACTGATTGCTTTTGTAGGAGAGGAGCTTCCTGCTA
AAAACACTAGGGATCGAGTCCTCATCTCCATGTCCAAGTGCTAATCCTTCAAAACCACCC
CCTTGCAAAAGAGTCCTTCTCCATAGCTTGTAAACCAACCAACCATCTCTCTCATACCCT
AAACCGCGGTTTAGTCTCCATGGAGACGTAATTTTTTCCACTTTTGCTTAGATTTCATAT
TAGGTTTTGCAGCCATATCGGCCTGGAAGTTTTTGTGAAGCGGGCATGCCATGATCTGCA
TTCCTCCCTCAAGGTTTTTAAGTTTCTTTCCTGGTCCTAGAATGTTAACGGTAACATTTT
CTAGCCCCATCAAAGAATGTTCTCAAGAATCTGCCATATACTCAGATTCAGATTTCTCTT
TCTTTTCTTACTTAACATAAGGAAGCCATCTACAAAAGA
   
Sequence Name 43-K117669-SALK_033970C-53us
GenBank Accession LN485359 [GenBank]
Sequencing primer gene specific primer, 53us: 5'-ACACTGGTGCCTTCTTTTGTAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117669-SALK_033970C-53us
TCTGATCTGAGTATATGGCAGATTCTTGAGAACATTCTTTGATCGGTCTAGAAAATGTTA
CCGTTAACATTCTAGGACCAGGAAAGAAACTTAAAAACCTTGAGGGAGGAATGCAGATCA
TGGCATGCCCGCTTCACAAAAACTTCCAGGCCGATATGGCTGCAAAACCTAATATGAAAT
CTAAGCAAAAGTGGAAAAAATTACGTCTCCATGGAGACTAAACCGCGGTTTAGGGTATGA
GAGAGATGGTTGGTTGGTTTACAAGCTATGGAGAAGGACTCTTTTGCAAGGGGGTGGTTT
TGAAGGATTAGCACTTGGACATGGAGATGAGGACTCGATCCCTAGTGTTTTTAGCAGGAA
GCTCCTCTCCTACAAAAGCAATCAGTCTTTTAAGACTCATTGTTTTTTCAAAGCCTAAGT
ATTGTATGATGAACAAAGAAGGTGCGAAGCTTACACTTTCGGAGAACTGTGGACTTGGTA
TACCGTCTGCCAAAGATCTGAACAATGGCGTGACCTGAATAGGCGAGCTGAACTTTTCTT
CGCCTGAGATGGGCATATTCTCATCAGGATATATTGTGGTGTAAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTTATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAG

Last Updated on 06.12.2018 16:11