Sequences from confirmation amplicon


Confirmation sequences for line SALK_034210 and hit At2g33320 are shown.
Line ID SALK_034210
Locus At2g33320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14119632
Primers used for PCR 39dr - LBb3, show primer details
   
Sequence Name 54-K111957-SALK_034210C-39dr
GenBank Accession LN485605 [GenBank]
Sequencing primer gene specific primer, 39dr: 5'-CAACTCCGAGAAGATCTAACATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K111957-SALK_034210C-39dr
ATCACTCGTCGGATCACTCCGATTAGATCTAACTACAGGCTACACCGATGAAATCGCCTA
TGCAATTTGGTACTCCGATGAGATCTAATTTAGCAGGAAGACCGGTTTTAACAGAATCCG
AATTAGGTCCGTCGCCATCAGAAGTAGCGCAGAAAATGGCGAAAGAGAGATCACAAGCTT
ACGAAACAGAGAGTTCGATTCTTAGTGAATGGAGCTTAGACGATGATAGTAACATCGAAG
GTCTCCGATCGAAACTTGAGAGATGGAGAACAGAGCTTCCGCCGCTTTACGATCTGGGAT
CGAGTCATCAAAGCAGCGATGTAGGAAGCGGCGCGATGGTTGTTGCTAATGTAGGTGGAG
GAAAAAGCTCGAGGAAGAAAACTCCGGCGGTGAAGAAGAAGCATAATCGCCGTCACACTG
AAGGAGGAGGTAACGGACTTTTCTCGTGTTTTAGTAATTTGTGCGGCGTGGAATTGACGC
TTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 50-K111956-SALK_034210C-R210
GenBank Accession LN485508 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K111956-SALK_034210C-R210
GCGTCATTCCCGCCGCAAAATTACTAAAACACGAGAAAAGTCCGTTACCTCCTCCTTCAG
TGTGACGGCGATTATGCTTCTTCTTCACCGCCGGAGTTTTCTTCCTCGAGCTTTTTCCTC
CACCTACATTAGCAACAACCATCGCGCCGCTTCCTACATCGCTGCTTTGATGACTCGATC
CCAGATCGTAAAGCGGCGGAAGCTCTGTTCTCCATCTCTCAAGTTTCGATCGGAGACCTT
CGATGTTACTATCATCGTCTAAGCTCCATTCACTAAGAATCGAACTCTCTGTTTCGTAAG
CTTGTGATCTCTCTTTCGCCATTTTCTGCGCTACTTCTGATGGCGACGGACCTAATTCGG
ATTCTGTTAAAACCGGTCTTCCTGCTAAATTAGATCTCATCGGAGTACCAAATTGCATAG
GCGATTTCATCGGTGTAGCCTTGTAGTTAGATCTAATCGGAGTTGATCCGACGATGTTGG
ATCGTAACGGAGTCGATCCGAGGATGTTAGATCTCTTCGGGAGTTG

Last Updated on 06.12.2018 16:11