Sequences from confirmation amplicon


Confirmation sequences for line SALK_035240c and hit At3g06830 are shown.
Line ID SALK_035240c
Locus At3g06830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2154709
Primers used for PCR 09gj - LBb3, show primer details
   
Sequence Name 19-K112064-SALK_035240C-09gj
GenBank Accession LN484814 [GenBank]
Sequencing primer gene specific primer, 09gj: 5'-TTGGAGATTGAAACTCACTGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112064-SALK_035240C-09gj
GAGCTGACTTGCGATCCGAATTGAGACTCCGGTGATTAGAGTCTTGTTTGGCCCATCGCC
GCTGTAAGTTACGTGAGGCATTTTCTTGGTGACCGTAACTTTCTCCTTGTAGATACCTTC
TTTGATGTGAATGATGAATGGCACTTTGTTGCCTTTAGGCACAGCATTTAGAGCATCGGT
GATAGTCTTGAACTGTCCAGTTCCGTCTTGAGCCACCACGGCATTAGCCTTCACAGGTCC
AGGTCCTCCTCCTTGAGCTGCCATGAGCCGTCGTGCCTCTGGTCCAACCCAAGTTGGGAT
ACTATCTTCTGTAGACAGAAGCTTTCTCGCGTACTTTGCTAAAGCTCCGGTTAGTCCTGG
TTTGAAGCATCACATTACTGTCCAATGATTCTGACTGGTTGGTTATGGATTAGTTTGGAG
ATCTCTTTAGTAAATTGAAAAGATACCTGTAAGGTTGGAGTTTGGGATAAGAGTGGAGAT
TCTAGTAACCATGGCAAGGCTATTGCTACTAAGCTCTCTTGATGTTTTGAAGATCTTGAG
CATGTCTTGCATAAGATTAGACTTAATAAATCAAATATACAAATTGTGGTGTAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGA
   
Sequence Name 03-K112049-SALK_035240C-R210
GenBank Accession LN484336 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112049-SALK_035240C-R210
CATTTGTTTACCACAATTTGTATATTTGATTTATTAAGTCTAATCTTATGCAAGACATGC
TCAAGATCTTCAAAACATCAAGAGAGCTTAGTAGCAATAGCCTTGCCATGGTTACTAGAA
TCTCCACTCTTATCCCAAACTCCAACCTTACAGGTATCTTTTCAATTTACTAAAGAGATC
TCCAAACTAATCCATAACCAACCAGTCAGAATCATTGGACAGTAATGTGATGCTTCAAAC
CAGGACTAACCGGAGCTTTAGCAAAGTACGCGAGAAAGCTTCTGTCTACAGAAGATAGTA
TCCCAACTTGGGTTGGACCAGAGGCACGACGGCTCATGGCAGCTCAAGGAGGAGGACCTG
GACCTGTGAAGGCTAATGCCGTGGTGGCTCAAGACGGAACTGGACAGTTCAAGACTATCA
CCGATGCTCTAAATGCTGTGCCTAAAGGCAACAAAGTGCCATTCATCATTCACATCAAAG
AAGGTATCTACAAGGAGAAAGTTACGGTCACCAAGAAAATGCCTCACGTAACTTTCATCG
GCGATGGGCCAAACAAGACTCTAATCACCGGTAGTCTCAATTTCGGTATCGGCAAGGTCA
AGACCTTCCTAACAGCCACAATCAGTGAGTTCNATTCTCC

Last Updated on 06.12.2018 16:11