Sequences from confirmation amplicon


Confirmation sequences for line SALK_035827c and hit At5g52240 are shown.
Line ID SALK_035827c
Locus At5g52240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21212911
Primers used for PCR 19ay - LBb3, show primer details
   
Sequence Name 52-K107479-SALK_035827C-19ay
GenBank Accession LN485552 [GenBank]
Sequencing primer gene specific primer, 19ay: 5'-TTTTTGGGAAACATTTAAGACTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K107479-SALK_035827C-19ay
AAAAAAATGTTATTATTGTAAATGTTAGGATTGGAAGATTTATCATGAGAAGAAAAGTGA
AAAATACTAATATAGTCAATTATGAATTTTCATAAATCTGAATTAAATATAATTTATTTT
TACTTAATATATAGTTTTAATTACTTTTTGTTTAAATTTATGTTATTAAATTTGGTATTT
GTATATCATGTTTAAAAGATTTAGAAGTATGATTTTATTCTATTTCTAGTGATTTAATTA
TTGAAACTTTGATTTTTAAAAAAGTTTATGTATTAATAAATTATTTGGATTGAAGGTTAT
ATCAATGTTATAAAATGTGTTTTAAAATCTGAAAAGTGGTTGATTTTTTCTTTTTAAATT
CTGCATCCACGAAACTTATTTTTCTTAAAACTTATTTTTCTTTTCGAGGTTATGATTCTT
TTTAGTTTTTTTTATCAAATTTCATGATGAAAATAACTGAATTGAACATCAAAGATTTTC
AGAAAATACTCTACTGCTTTTTTCTTAACTATATTCTAGCCATTTACATATCTAAGCTTG
ATGTCTTTTCTATTTCGTCGAAAATCTATCTATTTTACGGCAGAGACTTGAAACCCGAAT
TTTGACATATCAAAAACACTAACACAATCCCATGTACTACATTATCATTTGTATTTTACT
TATAGAAGAAAATAACTTTTTATTTAAAAATTGTATGGTAAAAATAAAGATTAGTTATCA
GGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTTCCCGCCTGGC
   
Sequence Name 68-K107480-SALK_035827C-LBb3
GenBank Accession LN485947 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K107480-SALK_035827C-LBb3
GGCAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCA
GCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCA
ATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTGATAACTA
ATCTTTATTTTTACCATACAATTTTTAAATAAAAAGTTATTTTCTTCTATAAGTAAAATA
CAAATGATAATGTAGTACATGGGATTGTGTTAGTGTTTTTGATATGTCAAAATTCGGGTT
TCAAGTCTCTGCCGTAAAATAGATAGATTTTCGACGAAATAGAAAAGACATCAAGCTTAG
ATATGTAAATGGCTAGAATATAGTTAAGAAAAAAGCAGTAGAGTATTTTCTGAAAATCTT
TGATGTTCAATTCAGTTATTTTCATCATGAAATTTGATAAAAAAAACTAAAAAGAATCAT
AACCTCGAAAAGAAAAATAAGTTTTAAGAAAAATAAGTTTCGTGGATGCAGAATTTAAAA
AGAAAAAATCAACCACTTTTCAGATTTTAAAACACATTTTATAACATTGATATAACCTTC
AATCCAAATAATTTATTAATACATAAACTTTTTTAAAAATCAAAGTTTCAATAATTAAAT
CACTAGAAATAGAATAAAATCATACTTCTAAATCTTTTAAACATGATATACAAATACCAA
ATTTAATAACATAAATTTAAACAAAAAGTAATTAAAACTATATATTAAGTAAAAATAAAT
TATATTTAATTCAGATTTATGAAAATTCATAATTGACTATATTAGTATTTTTCACTTTTC
TTCTCATGATAAATCTTCCAATCCTAACATTTACAATAATAACATTTTTTTTTTCTTTTG
GGTCAAAAAATGATAACAAAGTCTTAAATGTTTTCCAAAAA

Last Updated on 06.12.2018 16:11