Sequences from confirmation amplicon


Confirmation sequences for line SALK_036447c and hit At4g02650 are shown.
Line ID SALK_036447c
Locus At4g02650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1157221
Primers used for PCR 30mh - LBb3, show primer details
   
Sequence Name 84-K114279-SALK_036447C-30mh
GenBank Accession LN486274 [GenBank]
Sequencing primer gene specific primer, 30mh: 5'-TCTTGTCTTGAAACCACTTCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K114279-SALK_036447C-30mh
TTCTCTTCTTCTTTCTGTTCCGGTGCGGGTAATGCTTTGATGGAGTTTAAATCTTCTTGA
TTTTCTTGAATATACTCTGTTTTGCTCTCCTCCTCCTCAGATTTGTTAGACCTTTTTGAT
GATGATTTTGTTGTTTGTGCAGCTAAAGCTGATTTATCTCTTATAAACTCATCCATGAGA
TCAAGTTTCTTCTGTGTAATCTTTTCCAATTCTGGATACTCTGAAGATCTTGCAACTGCC
ATGTTCTTACACCATCCGTAAAACGGATCTAACTCGTCAAACTGTTTAGAAACTCGACAG
AAGATTTCATAGACTTTGATGGAATCATGAATGTCTAATTCCATGAATCGCTCTATTAAT
ACTCCCATGATCTCTGTTATATTGTAGTATAACTGAAAACTCTCTTTTACTATCGGATAC
ATAGCCACAATCACCACTCTGTTGTTCTTCGCATTACCTGTTGCAAAAGACCTAACTTAT
TAACCAAGAAATCCGAAAGTGGAAAAGCGTGTGTTCCAAGGAAATTAATTATTACCTGTT
GGACGACAAGCAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGATTGATGGTGGTTCCG
   
Sequence Name 70-K114278-SALK_036447C-R210
GenBank Accession LN486010 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K114278-SALK_036447C-R210
CAGGTAATAATTAATTTCCTTGGAACACCGCTTTTCCACTTTCGGATTTCTTGGTTAATA
AGTTAGGTCTTTTGCAACAGGTAATGCGAAGAACAACAGAGTGGTGATTGTGGCTATGTA
TCCGATAGTAAAAGAGAGTTTTCAGTTATACTACAATATAACAGAGATCATGGGAGTATT
AATAGAGCGATTCATGGAATTAGACATTCATGATTCCATCAAAGTCTATGAAATCTTCTG
TCGAGTTTCTAAACAGTTTGACGAGTTAGATCCGTTTTACGGATGGTGTAAGAACATGGC
AGTTGCAAGATCTTCAGAGTATCCAGAATTGGAAAAGATTACACAGAAGAAACTTGATCT
CATGGATGAGTTTATAAGAGATAAATCAGCTTTAGCTGCACAAACAACAAAATCATCATC
AAAAAGGTCTAACAAATCTGAGGAGGAGGAGAGCAAAACAGAGTATATTCAAGAAAATCA
AGAAGATTTAAACTCCATCAAAGCATTACCCGCACCGGAACAGAAAGAAGAAGAGGAAGA
AGAAGAGAAAATGGAAACAAAGAAAGATGTTGAAGAAGT

Last Updated on 06.12.2018 16:11