Sequences from confirmation amplicon


Confirmation sequences for line SALK_036792 and hit At4g27680 are shown.
Line ID SALK_036792
Locus At4g27680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13823081
Primers used for PCR 13ke - LBb3, show primer details
   
Sequence Name 55-K113814-SALK_036792C-R210
GenBank Accession LN485626 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K113814-SALK_036792C-R210
TCGTCATATCGGGAATCTTCTTCGGGATCCCGCTGCCATGAATCCTGAGATTCTGACTGT
AGGTTAATGATCTGAGAAACTAGAAGCTTCGAGATCCCACTTATCGCTGCCTGGACCTCA
TCTGGTTCCCTTGAGCCTCTCCAAGCCGAGGACTGCGAGCTCAATGAGTACTCGCCTGCT
GCAACTTGTGTCTTCTTTGACGTTGCAAGAACCTTCTCCAAATCTAACTGTGATAATGGC
CTAGGGTCCTGCAAACGTGACAAACTCATAACATCAGTACCTTGAAACTAGTGGATGCAN
ACACTGA
   
Sequence Name 68-K113815-SALK_036792C-13ke
GenBank Accession LN485956 [GenBank]
Sequencing primer gene specific primer, 13ke: 5'-TTCAGTGTTGCATCCACTAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113815-SALK_036792C-13ke
CCATTATCACAGTTAGATTTGGAGAAGGTTCTTGCAACGTCAAAGAAGACACAAGTTGCA
GCAGGCGAGTACTCATTGAGCTCGCAGTCCTCGGCTTGGAGAGGCTCAAGGGAACCAGAT
GAGGTCCAGGCAGCGATAAGTGGGATCTCGAAGCTTCTAGTTTCTCAGATCATTAACCTA
CAGTCAGAATCTCAGGATTCATGGCAGCGGGATCCCGAAGAAGATTCCCGATATTGACGA
ATAACACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCGAATCGGGCAAAA

Last Updated on 06.12.2018 16:11