Sequences from confirmation amplicon


Confirmation sequences for line SALK_037121c and hit At2g40830 are shown.
Line ID SALK_037121c
Locus At2g40830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17044452
Primers used for PCR 46et - LBb3, show primer details
   
Sequence Name 17-K111273-SALK_037121C-46et
GenBank Accession LN484744 [GenBank]
Sequencing primer gene specific primer, 46et: 5'-AGACTTTTGATCTCATGGATGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K111273-SALK_037121C-46et
GACAGAGAATCAGAGGAAGAACCATCAGTTCAGGTCCTGAAAACTTTCCTGGTCTGGCCC
CTTTGTTGATCTTTGGTGGTCAAGTCCCTTATAGACTAACTGGCGACAATGCAGTCGAAG
CCCTCTTCAATGGCGGCTCCCCTGGCATTGGCATCACACGTGGTAACACCGGCGACTACT
TCTTCGGTCCCGGCCTTGAAGAATTGTTCGAGCAGCTTTCAGCTGGCACTACTCGCCGAG
GCCCACCACCCGCACCGAGATCAGCAATAGACGCATTGCCAACTATCAAGATCGCGCAGA
GGCATCTTAGGTCATCGGACTCGAATTGTCCCGTGTGCAAAGACGAATTCGAACTGGGAT
CAGAAGCGAAACAGATGCCGTGTAACCACATCTATCATTCTGACTGCATTGTCCCGTGGC
TGGTTCAGCACAACTCTTGCCCGGTCTGTCGCCAAGAGCTACCATCAGCTAGCGGACCTT
CAAGCAGTCAAAACAGAACCACCCCTACCAGAAACTACAGAAGCAGTAGTAGTAGTAGTA
GTAGTAACAGTCGTGAGAACGGGAATGAAAACAAATTGCCGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 01-K111272-SALK_037121C-R210
GenBank Accession LN484275 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K111272-SALK_037121C-R210
CATTTGTTTTCTTCCCGTTCTCACGACTGTTACTACTACTACTACTACTACTGCTTCTGT
AGTTTCTGGTAGGGGTGGTTCTGTTTTGACTGCTTGAAGGTCCGCTAGCTGATGGTAGCT
CTTGGCGACAGACCGGGCAAGAGTTGTGCTGAACCAGCCACGGGACAATGCAGTCAGAAT
GATAGATGTGGTTACACGGCATCTGTTTCGCTTCTGATCCCAGTTCGAATTCGTCTTTGC
ACACGGGACAATTCGAGTCCGATGACCTAAGATGCCTCTGCGCGATCTTGATAGTTGGCA
ATGCGTCTATTGCTGATCTCGGTGCGGGTGGTGGGCCTCGGCGAGTAGTGCCAGCTGAAA
GCTGCTCGAACAATTCTTCAAGGCCGGGACCGAAGAAGTAGTCGCCGGTGTTACCACGTG
TGATGCCAATGCCAGGGGAGCCGCCATTGAAGAGGGCTTCGACTGCATTGTCGCCAGTTA
GTCTATAAGGGACTTGACCACCAAAGATCAACAAAGGGGCCAGACCAGGAAAGTTTTCAG
GACCTGAACTGATGGTTCTTCCTCTGATTTCTCTGTCGTGGCTCCTTTCAGCTAAGCGGT
TCCTCATAAACACAGAGAAAGCATCCATGAGACAAAAAGTC

Last Updated on 06.12.2018 16:11