Sequences from confirmation amplicon


Confirmation sequences for line SALK_037136 and hit At4g29920 are shown.
Line ID SALK_037136
Locus At4g29920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14632699
Primers used for PCR 54us - LBb3, show primer details
   
Sequence Name 07-K117668-SALK_037136C-R210
GenBank Accession LN484473 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117668-SALK_037136C-R210
TGGGAACTTGTCTACCCAACAGAATCCATGTTTCCTTTGTTGATTAGCTTCGGCTTTTAT
TTCTGTTCTTAATTTCTTCTATTAGAATTATATAAATATATTTTTGTTAGTATTATTTTT
CTTATTTCTTTTTGCTTTATAAGAAGAGAAAAGAAGAAAATTAATTATGACCCAAGAAAA
GTGGAACTTGACCGCATTGTTCTTTGTGTAAACATAAGTTGCCTCGCTTCATGATAGAAT
GTACTTTTTATATTCCTTTTTTAGGAATAGTGTTGTCACCTTTTCATTCCATTA
   
Sequence Name 44-K117669-SALK_037136C-54us
GenBank Accession LN485379 [GenBank]
Sequencing primer gene specific primer, 54us: 5'-TAATGGAATGAAAAGGTGACAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117669-SALK_037136C-54us
AAAGTCATTCTATATGAAGCGAGGCAACTTATGTTTACACAAAGAACAATGCGGTCAAGT
TCCACTTTTCTTGGGTCATAATTAATTTTCTTCTTTTCTCTTCTTATAAAGCAAAAAGAA
ATAAGAAAAATAATACTAACAAAAATATATTTATATAATTCTAATAGAAGAAATTAAGAA
CAGAAATAAAAGCCGAAGCTAATCAACAAAGGAAACATGGATTCTGTTGGGTAGACAAGT
TCCCATGATATAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAAT

Last Updated on 06.12.2018 16:11