Sequences from confirmation amplicon


Confirmation sequences for line SALK_037273c and hit At1g04750 are shown.
Line ID SALK_037273c
Locus At1g04750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1333308
Primers used for PCR AW09 - R204, show primer details
   
Sequence Name 43-K105819-SALK_037273C-AW09
GenBank Accession LN485345 [GenBank]
Sequencing primer gene specific primer, AW09: 5'-CCACCATATCGCTTGTTAAAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K105819-SALK_037273C-AW09
TGGGATTTGCCTCCCAGCAGAATCAACCGCAACAACACAATAGGCTGTACAACAGGAACA
AAATCAATCTCCTTGTCTTCCCTTACGGGATAATCAGGTGACATTGATTTAATATGAAAT
TGATATGGCTACAACAACTAAAGGATAGTCACAAAAGCTTACAATAAGACCCCAGATTTT
CATTGAAAAATGAAGCCAGCCAAAACCCATGACCTAGGAAACTATACGCAAATAAAGCAA
CCAGTAAAAACCCTGTCTCAGATCAATGTTGGTGTTCTTCCACATAGTATCAACCAAAGC
TACCACGGATTCTGTCCAAATCTGAGTGATTATACAGGTAAGACTCTACAGAGATTTATT
GCGTTTAATAAATATAGATCTAACCCTAAAAGCTTGGTTCGGTTCCTCAGTGAAATCAGA
CTACTCAGGATGCACAAATCCATACGAATCGATAGGTTAGGCAAATAAACCTAGAAACAA
ATCGAGGTAAGCACCGAATGGTGTAAGATTAAAACCTATGCATAGATCAAACGAAAAGTG
ACTTACTGAATCCATCTTCGACAAGGTAATTGAAGGTATGACCGTCGCAGTTGTAGGTGA
ACTTGTTGTTCGAAGACGCTTAAACAAATTGACAAATTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAGGGGGT
   
Sequence Name 59-K105820-SALK_037273C-R210
GenBank Accession LN485704 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K105820-SALK_037273C-R210
GTCATTTGTCATTTGTTTAGCGTCTTCGAACAACAAGTTCACCTACAACTGCGACGGTCA
TACCTTCAATTACCTTGTCGAAGATGGATTCAGTAAGTCACTTTTCGTTTGATCTATGCA
TAGGTTTTAATCTTACACCATTCGGTGCTTACCTCGATTTGTTTCTAGGTTTATTTGCCT
AACCTATCGATTCGTATGGATTTGTGCATCCTGAGTAGTCTGATTTCACTGAGGAACCGA
ACCAAGCTTTTAGGGTTAGATCTATATTTATTAAACGCAATAAATCTCTGTAGAGTCTTA
CCTGTATAATCACTCAGATTTGGACAGAATCCGTGGTAGCTTTGGTTGATACTATGTGGA
AGAACACCAACATTGATCTGAGACAGGGTTTTTACTGGTTGCTTTATTTGCGTATAGTTT
CCTAGGTCATGGGTTTTGGCTGGCTTCATTTTTCAATGAAAATCTGGGGTCTTATTGTAA
GCTTTTGTGACTATCCTTTAGTTGTTGTAGCCATATCAATTTCATATTAAATCAATGTCA
CCTGATTATCCCGTAAGGGAAGACAAGGAGATTGATTTTGTTCCTGTTGTACAGCCTATT
GTGTTGTTGCGGTTGATTCTGCTGGGAGGCAAATTCCCATGTCCTTTTTGGAAAGAGTAA
AAGAAGATTTTAACAA

Last Updated on 06.12.2018 16:11