Sequences from confirmation amplicon


Confirmation sequences for line SALK_037383c and hit At1g68490 are shown.
Line ID SALK_037383c
Locus At1g68490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25694901
Primers used for PCR CF44 - LBb3, show primer details
   
Sequence Name 21-K116922-SALK_037383C-CF44
GenBank Accession LN484892 [GenBank]
Sequencing primer gene specific primer, CF44: 5'-CAGTTGTTGAAAGAGATCAAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116922-SALK_037383C-CF44
CACCATCACCATCCTTCTCGATCTCTCCGTTGTTACTTCAGGTATGAATTCACTCACTTC
TCAAATTCAAAATCTCATGAGAATTCGTTTTCACTGTGAATTTTTACAAATTGCTAATTG
TGATTTGTTTCAGTCATCAATTGGAGCTTTGTGAATCCAAAGCAGAGACTGATATCTTAG
ATATCATACTCACCAAGGTATTTTTTGTTTGTTCTCTCTTGTTTTTTAGGTTATTGGTAG
GTGAGATTTGTTTATCAAGAAACCAATAAAAATCAGTAGCAAGTCGGTTTCGTCTAAAGA
TTTTTTTATTTCTCATAGATAGAGTAATCGTGTACGATTCTTGATTAAAGAAAACATGTG
ATCTTTCTTGTGTTTGTGACTTTGTCGATAAAGCTTTAGGATTAAGACGTCTTCGAGTTA
ACTCATTACAGCACATAGAGTGGGCCAATTCTCTAACCAATTTAAATAAATTATGCGTCC
TTTCTTTAAACCATTTAGCCAAACTTGATTTAGTCATCAAGAAACTGTATTTTTATGTTA
GTATATTCATGATATGTTTTTGTTGTGGTTATAGGATGGTTATGGTGCAGAACAAGTTAA
TAAGCAGGTAATAGACTCGCCGTCCCCGTTTTTATGTGGGTCGCCGCCGAGCAGAGTCGC
TAACCCATTAACACAGGATGCTCGATTTCGAGATGAGATTGTATCGGTTTCTTCAGTGAT
TCCGCCTCAGTTGGGTTTACCTCCTTCTTCATCTCCTTCTTCTTCCTCTGGGAGGAAAGG
AGGATGTGTTGTTAGAGGCAATTTTGGTAACAGCCCATAGGTTAGAGTTGAAAGGTTTGA
TTGTGATGTAAACACATTGACGCTTAGACCACTTAATAACACATTGCTGAACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
   
Sequence Name 10-K116921-SALK_037383C-R210
GenBank Accession LN484563 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K116921-SALK_037383C-R210
ACCACAATCAAACCCTTCAACTCTAACCTTTGGGCTGTTACCAAAATTGCCTCTAACAAC
ACATCCTCCTTTCCTCCCAGAGGAAGAAGAAGGAGATGAAGAAGGAGGTAAACCCAACTG
AGGCGGAATCACTGAAGAAACCGATACAATCTCATCTCGAAATCGAGCATCCTGTGTTAA
TGGGTTAGCGACTCTGCTCGGCGGCGACCCACATAAAAACGGGGACGGCGAGTCTATTAC
CTGCTTATTAACTTGTTCTGCACCATAACCATCCTATAACCACAACAAAAACATATCATG
AATATACTAACATAAAAATACAGTTTCTTGATGACTAAATCAAGTTTGGCTAAATGGTTT
AAAGAAAGGACGCATAATTTATTTAAATTGGTTAGAGAATTGGCCCACTCTATGTGCTGT
AATGAGTTAACTCGAAGACGTCTTAATCCTAAAGCTTTATCGACAAAGTCACAAACACAA
GAAAGATCACATGTTTTCTTTAATCAAGAATCGTACACGATTACTCTATCTATGAGAAAT
AAAAAAATCTTTAGACGAAACCGACTTGCTACTGATTTTTATTGGTTTCTTGATAAACAA
ATCTCACCTACCAATAACCTAAAAAACAAGAGAGAACAAACAAAAAATACCTTGGTGAGT
ATGATATCTAAGATATCAGTCTCTGCTTTGGATTCACAAAGCTCCAATTGATGACTGAAA
CAAATCACAATTAGCAATTTGTAAAAATTCACAGTGAAAACGAATTCTCATGAGATTTTG
AATTTGAGAAGTGAGTGAATTCATACCTGAAGTAACAACGGAGAGATCGAGAAGGATGGT
GATGGTTGTTACGGAGACCAATACGACGTGGTTTTGGACAAACGACGGTGGTTTGAT

Last Updated on 06.12.2018 16:11