Sequences from confirmation amplicon


Confirmation sequences for line SALK_037762 and hit At1g21440 are shown.
Line ID SALK_037762
Locus At1g21440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7503164
Primers used for PCR AD02 - LBb3, show primer details
   
Sequence Name 51-K107479-SALK_037762C-AD02
GenBank Accession LN485528 [GenBank]
Sequencing primer gene specific primer, AD02: 5'-CGATTGTGGACAGAGTAATCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107479-SALK_037762C-AD02
GTTTCTGAGTAGAGTACTTGGTTTCGAGCTCATACCATGAATCCAAGTTCACCAAACGGT
TAAACTCTTCGAAAGTTATCATCTTTTCTAAGTGGTCTTTGGTGGTTCCTTTCTCCTTGA
GAATCTTAAGAACATCGACAAGAGCTCGAGTCGACGCATAGAGCGAGGTAAGTGGGTGTG
CGATCAAGTGAAAACCCATTTCTTTAAGCTCATCAGGCGTATGCAATGGCGTGCGTCCAC
CTTCAAGCATGTTACACAGTCTGTAACCTTTTGTGCGCCTTCCGATTTCCTTAAGCTCGT
CATCGTCCCTCGGTGCCTCAACAAAGGAAGCATCAGCTCCTGCCTGCACATTTTTCCAAT
CTGTCTCTATTACCCACCATTATGTCTTCTCAAAAGTTAAAGCTTTAATAACACAACATG
ATTTAGTAGTAACCTCCATATAAAGATTAGCACGGTCAATGGCGTCTGAAAGTCCTGTTT
TTGCGGAAAGTGCACGAGCATCAGTCCTAGCGATTAGAAAAAAGTCAGCATCTCCAATAG
CGTCTCTTGCTGATGCTATTTTAGCAGCGTGTTCCTCCGCAGGTATGACCTGTTTCCAAA
TTAGAAAAATAATAATCATCTGCACTCATGGACTATAAAGTAATAAGTTTCAATGTATAG
ATTTTGGTAGCAAACGATGAGGGAATGGTACCAATACCTCTTTGCCACGCATATGTCCTA
CACAAATGACAAATTGAGAGAGCAAACGAGTTAAAACCATTATTGGACATTTTGACAATA
GCTTATAGGTTTGCAGAGGATGAAACAGAAAAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCAGCAGGCGAAATCCTGT
TGATGGTGGTC
   
Sequence Name 67-K107480-SALK_037762C-LBb3
GenBank Accession LN485920 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K107480-SALK_037762C-LBb3
GGCAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCA
GCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCA
ATGTGTTATTTTTCTGTTTCATCCTCTGCAAACCTATAAGCTATTGTCAAAATGTCCAAT
AATGGTTTTAACTCGTTTGCTCTCTCAATTTGTCATTTGTGTAGGACATATGCGTGGCAA
AGAGGTATTGGTACCATTCCCTCATCGTTTGCTACCAAAATCTATACATTGAAACTTATT
ACTTTATAGTCCATGAGTGCAGATGATTATTATTTTTCTAATTTGGAAACAGGTCATACC
TGCGGAGGAACACGCTGCTAAAATAGCATCAGCAAGAGACGCTATTGGAGATGCTGACTT
TTTTCTAATCGCTAGGACTGATGCTCGTGCACTTTCCGCAAAAACAGGACTTTCAGACGC
CATTGACCGTGCTAATCTTTATATGGAGGTTACTACTAAATCATGTTGTGTTATTAAAGC
TTTAACTTTTGAGAAGACATAATGGTGGGTAATAGAGACAGATTGGAAAAATGTGCAGGC
AGGAGCTGATGCTTCCTTTGTTGAGGCACCGAGGGACGATGACGAGCTTAAGGAAATCGG
AAGGCGCACAAAAGGTTACAGACTGTGTAACATGCTTGAAGGTGGACGCACGCCATTGCA
TACGCCTGATGAGCTTAAAGAAATGGGTTTTCACTTGATCGCACACCCACTTACCTCGCT
CTATGCGTCGACTCGAGCTCTTGTCGATGTTCTTAAGATTCTCAAGGAGAAAGGAACCAC
CAAAGACCACTTAGAAAAGATGATAACTTTCGAAGAGTTTAACCGTTTGGTGAACTTGGA
TTCATGGTATGAGCTCGAAACCAAGTACTCTAACCTCAGAAACGCTCTAGGGGAAACAAA
ATAATGAGAATCTGATA

Last Updated on 06.12.2018 16:11