Sequences from confirmation amplicon


Confirmation sequences for line SALK_038477c and hit At3g59990 are shown.
Line ID SALK_038477c
Locus At3g59990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22156317
Primers used for PCR 58fr - LBb3, show primer details
   
Sequence Name 09-K113018-SALK_038477C-58fr
GenBank Accession LN484522 [GenBank]
Sequencing primer gene specific primer, 58fr: 5'-TTACGCTGCTGATGGTTATATACG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113018-SALK_038477C-58fr
ATGCGCTCTGTCNCCTCGAGGAGACGACAGTAACGAGGGAATTCGCGGCGGACAGTAAGC
GATGAGATTTGAACGGCGAGAACTCCAGCCTTCACTTTCAGACCACCGGTAAAAACAGCT
ATCGTCGCCGCCATGGAAATGCCTCGAGTAGCTCAAAAGTCTATTGGCTAAGATCTCTGT
GTGATCCCAGTGTTCTCTCTTCTTTACCATATCTGTATTTATATATCTTTTTATATGATC
TTGTCAAATTAGTAGCTTTACCAATTCTCCAAAGCAAGGAGCTTTATCAATCAACTTCTA
GTAAGATTGATGAGCAAATTCATGTTCATGGCAAATACATAGTTTTTTTTTAACAGAGGC
ACTGAAGCTTTTTATAAAAGGATTCAAAGCTTGTGTTAACGACGACAAAAGAAACAAGCA
AGTGTACAATCTCGACAACAGTATGAGATAATAAAGGTAAACAGAGCAAAACAGAGGATC
TGAATCAGTAGTCGTCTCCCTTGGAGAGAACTTCTTTGCAAGTAGGTCGCAGTAAAATGG
TGTGTTCAAACTGTGATACATAGCTTCCTTTCACATCACACAGAGGAGGATACGGCTGAA
ATAAAACAGAGAGCAAGATTTAAGTGTATTTAGATCTCAGGATAGATCCGAGATAAATGA
ATCATCATCTAGATTGTGCAAGAAATTACCTGAACAATGCCAGAGTCACAAAGATTCTTT
AGAGCCATTAAGTATTTGGTTTCACCAATGCGGTCCAAATAACGTCTGCAGAAGGCGAGA
GTCGAGAAATTCTTGTTAATGGTTGCAAGGAGTTGTTTTGCTCTAGGCAACCTCAAGGGG
ACGTGGCCAGCGTCAAAGTTCTTCATGTAATGGCTACATTCAGGATATATTGTGGTGTAA
ACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTTATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCTCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGNCAGCGGTCCACGCTGGTTTGCCC
   
Sequence Name 01-K113017-SALK_038477C-R210
GenBank Accession LN484282 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113017-SALK_038477C-R210
CTGCACTCTCTCAGGGCCAGGCGGTGAAGGGCAACCTTATAATGCCCGTCTCACTGGTGA
AAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGC
GTCAATTTGTTTACACCACAATATATCCTGAATGTAGCCATTACATGAAGAACTTTGACG
CTGGCCACGTCCCCTTGAGGTTGCCTAGAGCAAAACAACTCCTTGCAACCATTAACAAGA
ATTTCTCGACTCTCGCCTTCTGCAGACGTTATTTGGACCGCATTGGTGAAACCAAATACT
TAATGGCTCTAAAGAATCTTTGTGACTCTGGCATTGTTCAGGTAATTTCTTGCACAATCT
AGATGATGATTCATTTATCTCGGATCTATCCTGAGATCTAAATACACTTAAATCTTGCTC
TCTGTTTTATTTCAGCCGTATCCTCCTCTGTGTGATGTGAAAGGAAGCTATGTATCACAG
TTTGAACACACCATTTTACTGCGACCTACTTGCAAAGAAGTTCTCTCCAAGGGAGACGAC
TACTGATTCAGATCCTCTGTTTTGCTCTGTTTACCTTTATTATCTCATACTGTTGTCGAG
ATTGTACACTTGCTTGTTTCTTTTGTCGTCGTTAACACAAGCTTTGAATCCTTTTATAAA
AAGCTTCAGTGCCTCTGTTAAAAAAAAACTATGTATTTGCCATGAACATGAATTTGCTCA
TCAATCTTACTAGAAGTTGATTGATAAAGCTCCTTGCTTTGGAGAATTGGTAAAGCTACT
AATTTGACAAGATCATATAAAAAGATATATAAATACAGATATGGTAAAGAAGAGAGAACA
CTGGGATCACACAGAGATCTTAGCCAATAGACTTTTGAGCTACTCGAGGCATTTCCCTGG
CGGCGACGATAGCTGTTTTTACCGGTGGTCTGAAAGTGAAGGCTGGAGTTCTCCCCGTTC
AAATCTCATCGCTTCCTCTCCGCCGCGAATTCCCTCGTTACTTGTCGTCTTCC

Last Updated on 06.12.2018 16:11