Sequences from confirmation amplicon


Confirmation sequences for line SALK_039631c and hit At5g59750 are shown.
Line ID SALK_039631c
Locus At5g59750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24073560
Primers used for PCR 52aq - R204, show primer details
   
Sequence Name 52-K107203-SALK_039631C-52aq
GenBank Accession LN485550 [GenBank]
Sequencing primer gene specific primer, 52aq: 5'-ATTTTGCATCCTGTAACCAAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K107203-SALK_039631C-52aq
TGAACCCTCTTGAAACTATATCCAATCGTTACCACAGTGATGGTGAAAGTTGGAGCAGAG
GAATCTTCGTCTTCCATTTCAGGTGACATAAGTGTAAGGCTTAATCTTTCAAGGTTTTCT
TTCTTCATACCAACTGAGACAATACCTGAACCATTCTTAATCAGAAAAGCTATGTCTTTT
GGACTCGTAAGAGTTGCAGCCATGATCAGATTCCCTTCAACATCTCCGGTTTCATCGTCA
ACAACGATCACAAACTGAAACATAAGTGACAATTTTTTAGTAAGACCAAATCTATACAAC
TGTAAAAACAGATCATCATAATCACTGGTCTTCAAGAAAATCGTCAAAGAACAAACCTTT
CCTTTGCGTAGAGCTTGAAGAGCAAGTTCAATAGAGGAATAACCTTGAGTAGGACAATCT
GGATCACCCTCTGCGTCACTCACGAAGAAATCATCAGTCTCAGGCGTGATTTCCGCGTCA
ACTGTCTCAAACGAAGCAGAATCAAACAACGACCCGTCGGTTTTCCTAACAGGATTTTGT
TCCGCTTAGACAACTTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCA
   
Sequence Name 44-K107202-SALK_039631C-R210
GenBank Accession LN485366 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107202-SALK_039631C-R210
CGTCAGTTGTCTAGCGGAACAAAATCCTGTTAGGAAAACCGACGGGTCGTTGTTTGATTC
TGCTTCGTTTGAGACAGTTGACGCGGAAATCACGCCTGAGACTGATGATTTCTTCGTGAG
TGACGCAGAGGGTGATCCAGATTGTCCTACTCAAGGTTATTCCTCTATTGAACTTGCTCT
TCAAGCTCTACGCAAAGGAAAGGTTTGTTCTTTGACGATTTTCTTGAAGACCAGTGATTA
TGATGATCTGTTTTTACAGTTGTATAGATTTGGTCTTACTAAAAAATTGTCACTTATGTT
TCAGTTTGTGATCGTTGTTGACGATGAAACCGGAGATGTTGAAGGGAATCTGATCATGGC
TGCAACTCTTACGAGTCCAAAAGACATAGCTTTTCTGATTAAGAATGGTTCAGGTATTGT
CTCAGTTGGTATGAAGAAAGAAAACCTTGAAAGATTAAGCCTTACACTTATGTCACCTGA
AATGGAAGACGAAGATTCCTCTGCTCCAACTTTCACCATCACTGTGGTAACGATTTGGAT
ATAGTTCTCAAGAGGGTTTCATTGTGTCTATGTTCTGATAAAGTTCTTGGTTACAGGATG
CAAAA

Last Updated on 06.12.2018 16:11