Sequences from confirmation amplicon


Confirmation sequences for line SALK_040027 and hit At1g52370 are shown.
Line ID SALK_040027
Locus At1g52370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19507155
Primers used for PCR 46sf - LBb3, show primer details
   
Sequence Name 74-K116811-SALK_040027C-46sf
GenBank Accession LN486115 [GenBank]
Sequencing primer gene specific primer, 46sf: 5'-AAGAAACATCGATTCAGAAGCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K116811-SALK_040027C-46sf
ATAGGTTGATCTCAGAGACTCTGCAAATAACCTAGTTCAACAGCAAAAATTGAAAAAAAA
AAAAAATCTTCCCCCCCTTAATTAAAACCCCCAAACCTTTTTTTAAACCAAAATCCCAAA
ATACCCCCGGACTTTTGAAATTTTCCCTAAAAACCCAATAGTTCCAAAATGGTTTTACCT
TTTTCTAACCCGAATGGATCAAACCTAACCGGTTCCAAGGGGTAAAATTTCCCCCCCCTG
GGGAAAAGGGGGATCCCCCTTTTGGGCGGGATATTTGGGGGGGAAAACAATTGGCCCCTA
AAACACTTTAAAAACCCTTGGGGGACTTTTTAAAGGACCGGGGGGGGTTTTTCTTTTCCC
CGGGGAAAGGGGCAACGGCTGATGCCCCTTCCCCCCCGGGCCCTAAAAAATTTCCACCAG
GCGGCCCCCCCGGGTTTCCCCCACCGGGCAAAAACCCGGTTGGAGGGGGGTCCCAA
   
Sequence Name 74-K116810-SALK_040027C-R210
GenBank Accession LN486114 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K116810-SALK_040027C-R210
ACCACAATATATCCTGCCATATGTGGAATCCCCTTTCTCACAAGGTGGTGAATATTATAC
CATTTGAAGCTGTTTAGTTTTGATCATTTCGGTTTAGATAATGTTAAATCATTTCTGAAT
CTATTGTGTTCTTAGTGAAATTTCAAAATGTCAGGTGTTATCTTGGATTTTTGTTTTAAT
ATAGTGTTTGTGTGTTTGAGTTGAGTGGGTGATGATTTTTTTTTTTTTCATTTTTGGCGG
TGAAACAGGGTTTTTTCCAAATTCCTTTGAAATCACCCTTTCCCTCAAAACCCCTGTTTT
ACCCTCACCAACACCGGGAAAGGCTTTGAAATCGATTTTTTTTA

Last Updated on 06.12.2018 16:11