Sequences from confirmation amplicon


Confirmation sequences for line SALK_040054c and hit At1g76970 are shown.
Line ID SALK_040054c
Locus At1g76970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28923144
Primers used for PCR 33cv - LBb3, show primer details
   
Sequence Name 11-K109155-SALK_040054C-33cv
GenBank Accession LN484577 [GenBank]
Sequencing primer gene specific primer, 33cv: 5'-TAATTTCCTATTTTATGTGGGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K109155-SALK_040054C-33cv
GGTTTAAAGCTCTGTTTCTCCTAGCAAAAGGTAAATTAATAAAGTCCCGAGAATTTACCC
AAAAAATAATGATCCGTGTGGAACTTAACTAAGGGGGTGTTATTAGTTTATAATATGCAA
AAGATATCTGATATTACGATTTTGTGTGTTAAAAGTATTTTAAAGCTGTGTCCTTGGAGT
GAAGTTAGAGAAGTAAAACATATGGTTGTTGGTATTATCAGATACTATCTCTAAATAATA
AGGCACACTCTAATATCATCATCACTCTCTGAAAACCCAAAAGAAAAAAAAAACTTGAAT
GGTAAAAAGCCACAAACAAAAGACTCAGAGCGACCTATTATTATTGTTGGCTTTGGAGGA
CCTGGTTTTGGCGAACTCAACCAGGTCTTTGAAAAGCAAATCCTCTGGTTTCTCTTCTTT
CTGCGGTTCACTACTTGTCAAAGAAAGGTTCCGGGTCTGTCCCTCGTAAGAGGAATCTGA
GCCGCTACTCGAGTGGTGATGCTCAAAGAACTGTTGCCTCTGGTTGTGCCTTGAAGGCGG
AGGTGGAAGATTCCTAATTACCTCAGAGGATTTGCTTTGTTGTGGACTTGCATCATCGAA
AACNGGAGAAGAAGAGGAAGAAGAAGTAAGGTTATTGTAAACAAATTGACGCTTAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAACTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCC
   
Sequence Name 03-K109154-SALK_040054C-R210
GenBank Accession LN484330 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K109154-SALK_040054C-R210
GTCATTTGTTACAATAACCTTACTTCTTCTTCCTCTTCTTCTCCTGTTTTCGATGATGCA
AGTCCACAACAAAGCAAATCCTCTGAGGTAATTAGGAATCTTCCACCTCCGCCTTCAAGG
CACAACCAGAGGCAACAGTTCTTTGAGCATCACCACTCGAGTAGCGGCTCAGATTCCTCT
TACGAGGGACAGACCCGGAACCTTTCTTTGACAAGTAGTGAACCGCAGAAAGAAGAGAAA
CCAGAGGATTTGCTTTTCAAAGACCTGGTTGAGTTCGCCAAAACCAGGTCCTCCAAAGCC
AACAATAATAATAGGTCGCTCTGAGTCTTTTGTTTGTGGCTTTTTACCATTCAAGTTTTT
TTTTTCTTTTGGGTTTTCAAAGAGTGATGATGATATTAGAGTGTGCCTTATTATTTAGAG
ATAGTATCTGATAATACCAACAACCATATGTTTTACTTCTCTAACTTCACTCCAAGGACA
CAGCTTTAAAATACTTTTAACACACAAAATCGTAATATCAGATATCTTTTGCATATTATA
AACTAATAACACCCCCTTAGTTAAGTTCCACACGGATCATTATTTTTTGGGTAAATTCTC
GGGACTTTATTAATTTACCTTTTGCTAGGAGAAACAGAGCTTTTAAAACCCAAAATAGCT
AACAACATATATTTTTACTGCCCCACATAAAAAGG

Last Updated on 06.12.2018 16:11