Sequences from confirmation amplicon


Confirmation sequences for line SALK_040274c and hit At4g05000 are shown.
Line ID SALK_040274c
Locus At4g05000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2564437
Primers used for PCR 17td - LBb3, show primer details
   
Sequence Name 75-K116811-SALK_040274C-17td
GenBank Accession LN486137 [GenBank]
Sequencing primer gene specific primer, 17td: 5'-AGGTCAAATTATGGAACGACAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K116811-SALK_040274C-17td
TTTTTGCATCATCAAAGCCACAGAGAAGCTAGAGAAAGCTTATATAAGAGACCTAATCAA
TCCTTCAGAGTACGAATCTGAATGCCAGAAACTCATTGTCCACTTCAAAACTTTATCCGC
TACACTCAAAGACACGGTGCCAAACATTGAAAGATTTGCAGACACATACAAAATGGACTG
TCCAGCTGCTCTATACCGCCTGGTGACATCAGGTCTTCCTGCCACAGTGGAACACCGAGC
TACCGTTGCAGCATCTACGTCGAACTCTGCATCCATTGTTGCTGAATGTGTACAGAATTT
CATCACTTCAATGGATTCTTTGAAACTAAACATGGTGGCTGTCGATCAGGTTTATCCTCT
CTTGTCTGATCTATCTGCTTCTCTCAACAAACTGAGTATTCTACCGCCCGATTTCGAAGG
TGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTTCCG
   
Sequence Name 75-K116810-SALK_040274C-R210
GenBank Accession LN486136 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K116810-SALK_040274C-R210
ACCACCTTCGAATCGGGCGGTAGAATACTCAGTTTGTTGAGAGAAGCAGATAGATCAGAC
AAGAGAGGATAAACCTGATCGACAGCCACCATGTTTAGTTTCAAAGAATCCATTGAAGTG
ATGAAATTCTGTACACATTCAGCAACAATGGATGCAGAGTTCGACGTAGATGCTGCAACG
GTAGCTCGGTGTTCCACTGTGGCAGGAAGACCTGATGTCACCAGGCGGTATAGAGCAGCT
GGACAGTCCATTTTGTATGTGTCTGCAAATCTTTCAATGTTTGGCACCGTGTCTTTGAGT
GTAGCGGATAAAGTTTTGAAGTGGACAATGAGTTTCTGGCATTCAGATTCGTACTCTGAA
GGATTGATTAGGTCTCTTATATAAGCTTTCTCTAGCTTCTCTGTGGCTTTGATGATTGCA
AAAAGCTCTGCAAAATTCTCATACATCTCTCTTTCGCGTTTGTCGTTCCTAATTTTGACC
T

Last Updated on 06.12.2018 16:11