Sequences from confirmation amplicon


Confirmation sequences for line SALK_040526c and hit At2g35765 are shown.
Line ID SALK_040526c
Locus At2g35765 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15033475
Primers used for PCR 18td - LBb3, show primer details
   
Sequence Name 46-K116873w-SALK_040526C-18td
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18td: 5'-CCTCTTCGCTCATAAACAAATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K116873w-SALK_040526C-18td
ACAACAACAACAACAAAAGAAGTTATATAACATAACGTTGAACCATGTGAATTAGAAACT
AGGAGAAAGATTTAAAACAACATACTTAGCTTTATCACCAAACCAGTGGTAGACGGATTT
GACGGTAGAGGAGACGGAGTCCACGACAGGACGGGCATGTTCATCGAAGAATTTGGTAAA
GTCGGTTGCGGCTGAGTCTATCCTCTGTCCGATGGTTGGTGTTTCCTCGGCAGAAACCAA
CACGGACATAAGCAACATCAAAGCCATCACCAAAGCCACCTTCTTCTCCATGATCCTGTC
CGTTAACGTAGATTCTTCCTTCTTCTATTTTCTTTCTTTGTGAAAGAGGTGTCTTAAATA
AATGACTTGAGGGAACAAAATGTAAAACAATGACATCCTCGTGAGTATGTCTATATATAA
CTTGCTCTTTCTGGCCACAGTCTATATATATTGTGGTGTAAACAAATTGACGCTTAGACA
ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
AGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 43-K116872w-SALK_040526C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K116872w-SALK_040526C-R210
CACAATATATATAGACTGTGGCCAGAAAGAGCAAGTTATATATAGACATACTCACGAGGA
TGTCATTGTTTTACATTTTGTTCCCTCAAGTCATTTATTTAAGACACCTCTTTCACAAAG
AAAGAAAATAGAAGAAGGAAGAATCTACGTTAACGGACAGGATCATGGAGAAGAAGGTGG
CTTTGGTGATGGCTTTGATGTTGCTTATGTCCGTGTTGGTTTCTGCCGAGGAAACACCAA
CCATCGGACAGAGGATAGACTCAGCCGCAACCGACTTTACCAAATTCTTCGATGAACATG
CCCGTCCTGTCGTGGACTCCGTCTCCTCTACCGTCAAATCCGTCTACCACTGGTTTGGTG
ATAAAGCTAAGTATGTTGTTTTAAATCTTTCTCCTAGTTTCTAATTCACATGGTTCAACG
TTATGTTATATAACTTCTTTTTGTTGTTGTTGTTTGTTGTATATTTGTAGGGAATGGGGA
CTCTAGAGATTTGTTTATACGCGAAGAGG

Last Updated on 06.12.2018 16:11