Sequences from confirmation amplicon


Confirmation sequences for line SALK_040744c and hit At5g61570 are shown.
Line ID SALK_040744c
Locus At5g61570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24758641
Primers used for PCR 04as - R204, show primer details
   
Sequence Name 51-K107035-SALK_040744C-R210
GenBank Accession LN485524 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107035-SALK_040744C-R210
ATTGATTCTTCGTCGAATCTCCTTACGAGAAACAAGAATTTTCCGATAACGGATCCGAAA
CAGAGGAGGAGCTTATCATCTTCAACGGTGGAGAAGATCTAACGATCTGCGATATACTCG
ATGCTCCAGGAGAAGTCATCGGAAAATCAAGCTACGGTACATTATACAAAGCGACGTTAC
AAAGAAGTGGGAAAGTTAGAGTTCTTAGGTTCCTTCGACCTTTATGTGCTGTGAATTCAG
ATTCTAAGGAGTTTAATGGCGTCATTGAGTCACTTGGGTTTGTTCGACATGATAATCTCG
TTCCTCTGTTAGGGTTTTA
   
Sequence Name 59-K107036-SALK_040744C-04as
GenBank Accession LN485707 [GenBank]
Sequencing primer gene specific primer, 04as: 5'-TAAAACCCTAACAGAGGAACGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K107036-SALK_040744C-04as
CCAGCGACTCATGACGCCATTAAACTCCTTAGAATCTGAATTCACAGCACATAAAGGTCG
AAGGAACCTAAGAACTCTAACTTTCCCACTTCTTTGTAACGTCGCTTTGTATAATGTACC
GTAGCTTGATTTTCCGATGACTTCTCCTGGAGCATCGAGTATATCGCAGATCGTTAGATC
TTCTCCACCGTTGAAGATGATAAGCTCCTCCTCTGTTTCGGATCCGTTATCGGAAAATTC
TTGTTTCTCGTAAGGAGATTCGACGAAGAATCAATGTGTTATGACACTGCAAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
G

Last Updated on 06.12.2018 16:11