Sequences from confirmation amplicon


Confirmation sequences for line SALK_041504c and hit At2g26580 are shown.
Line ID SALK_041504c
Locus At2g26580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11305096
Primers used for PCR 25ux - LBb3, show primer details
   
Sequence Name 20-K117668-SALK_041504C-R210
GenBank Accession LN484865 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117668-SALK_041504C-R210
CATTTGTTTACCACAATATATTGTGGATACATACATGATTGCTACTCAATCCTATTGATT
CACCATTTTTGGTCTGAAAAATCAAAGAAAAGGCCAAGTGATTAAATACTCTAAACTTCT
TAGCATTTATACTTTTATCAGGCAACAAACTATGCAGTACCAGAGTATGGATCCTCCTCA
AGAAGCCACACAAAAATCCCTTCAAGAATTTCAACTCGTACCATAACTGAGCAACGGATT
GTAAACCGTCGTAAGTATTGCTTACTATATAAACCTAAAGAAGTAAACATGAAAAGTAAT
TTGTGTACTGTATATGCTAAAATTATTATATTGTCTTTCAGCTCCGGAGAAGCGGCAGCG
AGTACCTTCTGCGTACAATCAATTCATAAAGTAACAAAAACATTTTAGACATACATATAT
CTATATATCTATATAATGTTCAAATCTGTTAAGAAAGAGGTTATTGATTTGTGTGTTTAT
ATTAAAACCTTTGAAGAGAGGAAATTCAGAGGATTAAGGCGAATAATCCAGACATAAGCC
ACAGAGAAGCATTCAGCACTGCTGCC
   
Sequence Name 57-K117669-SALK_041504C-25ux
GenBank Accession LN485671 [GenBank]
Sequencing primer gene specific primer, 25ux: 5'-AAAACTTACATTCTTGGCAGCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K117669-SALK_041504C-25ux
GATATTCGCCTAATCCTCTGAATTTCCTCTCTTCAAGGTTTTAATATAAACACACAAATC
AATAACCTCTTTCTTAACAGATTTGAACATTATATAGATATATAGATATATGTATGTCTA
AAATGTTTTTGTTACTTTATGAATTGATTGTACGCAGAAGGTACTCGCTGCCGCTTCTCC
GGAGCTGAAAGACAATATAATAATTTTAGCATATACAGTACACAAATTACTTTTCATGTT
TACTTCTTTAGGTTTATATAGTAAGCAATACTTACGACGGTTTACAATCCGTTGCTCAGT
TATGGTACGAGTTGAAATTCTTGAAGGGATTTTTGTGTGGCTTCTTGAGGAGGATCCATA
CTCTGGTACTGCATAGTTTGTTGCCTGATAAAAGTATAAATGCTAAGAAGTTTAGAGTAT
TTAATCACTTGGCCTTTTCTTTGATTTTTCAGACCAAAAATGGTGAATCAATAGGATTGA
GTAGCAATCATGTATGTATCCACAATATATTGTGGTGTAAACAAATTGACGCTTAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC

Last Updated on 06.12.2018 16:11