Sequences from confirmation amplicon


Confirmation sequences for line SALK_041720 and hit At3g61580 are shown.
Line ID SALK_041720
Locus At3g61580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22786621
Primers used for PCR 55bk - LBb3, show primer details
   
Sequence Name 26-K107841-SALK_041720C-55bk
GenBank Accession LN485023 [GenBank]
Sequencing primer gene specific primer, 55bk: 5'-TAAGGTAACACGTGGTCTGTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K107841-SALK_041720C-55bk
GTTTCCTCCTTTCTATTACCATAACTAACCATTTTCTTAATTGCCCAATAATGGTCCCTC
TTTACCAATTCTGATATACCACCCAAAATTAACATCAAACCCAATATTTAAAGTCCCACA
CGTGAGAACGACTGACGTCGGCACGCTTTAGCCACGTAATTTCGGTCTCCAAAAATTCAA
TATTAATCCGCGAAAATTCACTAAATATAAAACAAACCCATAATATAAGGAGGATAAGGA
AGGAGTCGAAGATAAGCGGAGAGAGAGAGAGAGACAGAGAGAGATTCAAAAATCCGATTC
CAGATCCATTCCTGGGCAAACAAAGGTTGGTGTTTCTCTAATCTCAAAGCTTTTTTCAAA
TTCGGAAAAAGCAAATCGTGGGAAGAGATTCATCTTCTCTCTGTGCGTTCATCGGATCTC
GGAGCTTTTGGTTCGTCGTCAATGGCGGAAGAGACGGAGAAAAAGTACATTACGAACGAA
GATCTTAAAAAACACAACAAATCTGGAGATCTATGGATCGCGATTCAAGGCAAGGTCTAC
AACGTCTCCGATTGGATTAAAACTCATCCCGGAGGCGACACGGTGATTCTCAATCTCGTT
GGTCAAGACGTCACCGATGCTTTCATCGCATTTCATCCCGGAACCGCTTGGCACCATCTC
GACCATCTCTTCACCGGTTACCACATCAGAGATTTCCAAGTCTCCGAAGTCTCACGCGAT
TACCGTCGTATGGCTGCCGAGTTTCGTAAACTCGGTCTCTTCGAAAACAAAGGTCACGTT
ACTCTCTACACTCTAGCCTTCGTCGCCGCCTTCGTTTCCCAAGATATATTGTGGTGTAAA
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGGGG
   
Sequence Name 27-K107842-SALK_041720C-R210
GenBank Accession LN485053 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K107842-SALK_041720C-R210
GCGTCATTTGTTTACCACAATATATCCTGGGAAACGAAGGCGGCGACGAAGGCTAGAGTG
TAGAGAGTAACGTGACCTTTGTTTTCGAAGAGACCGAGTTTACGAAACTCGGCAGCCATA
CGACGGTAATCGCGTGAGACTTCGGAGACTTGGAAATCTCTGATGTGGTAACCGGTGAAG
AGATGGTCGAGATGGTGCCAAGCGGTTCCGGGATGAAATGCGATGAAAGCATCGGTGACG
TCTTGACCAACGAGATTGAGAATCACCGTGTCGCCTCCGGGATGAGTTTTAATCCAATCG
GAGACGTTGTAGACCTTGCCTTGAATCGCGATCCATAGATCTCCAGATTTGTTGTGTTTT
TTAAGATCTTCGTTCGTAATGTACTTTTTCTCCGTCTCTTCCGCCATTGACGACGAACCA
AAAGCTCCGAGATCCGATGAACGCACAGAGAGAAGATGAATCTCTTCCCACGATTTGCTT
TTTCCGAATTTGAAAAAAGCTTTGAGATTAGAGAAACACCAACCTTTGTTTGCCCAGGAA
TGGATCTGGAATCGGATTTTTGAATCTCTCTCTGTCTCTCTCTCTCTCTCCGCTTATCTT
CGACTCCTTCCTTATCCTCCTTATATTATGGGTTTGTTTTATATTTAGTGAATTTTCGCG
GATTAATATTGAATTTTTGGAGACCGAAATTACGTGGCTAAAGCGTGCCGACGTCAGTCG
TTCTCACGTGTGGGACTTTAAATATTGGGTTTGATGTTAATTTTGGGTGGTATATCAGAA
TTGGTAAAGAGGGACCATTTATTGGGCAATTAAGAAAATGGTTAGTTATGGTAATTAGAA
AGGAGGAAACTTAGTGCTGTCCCTCGTAAGTAATCAGGAAATGCAAG

Last Updated on 06.12.2018 16:11