Sequences from confirmation amplicon


Confirmation sequences for line SALK_042206c and hit At4g33700 are shown.
Line ID SALK_042206c
Locus At4g33700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16176603
Primers used for PCR 66kh - LBb3, show primer details
   
Sequence Name 04-K114130-SALK_042206C-R210
GenBank Accession LN484374 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114130-SALK_042206C-R210
TTTGTTACAGCCAGCAATAATTAATCCGTGAAAAAGAATTTTTGTTTTTAACTTATATAA
GATTCCTTACCTGCAGAAGTTCTTCAATGACATCCTCCATCGTAATGATTCCGACTGCTT
CTTCTTCCTCAGCAAGTTTAGGCAGCGGATTGCCATTCAATTGCAGGATGTCTGCATCAT
TGTCTTTTGACCACTTCTTGCTCTTTGACCCTCCTTTAAACGAACTTGCTCGATTCGGAA
AGCTCTTCCACTTTTGAAGCGATCTTTTTGTCCTTAACATTCTCTCCTGAGGAGTAGGAG
TTCCCTCACTATCCACATCCACTCGGGCTTCCTTAACACTCCCTATTAGAAACCAAAGCA
AAGATTCTCAATACTTGTAACTCAGCAAGACATTGTTTTGTCGATTAATGAAGCAGTTTA
CCATTTTTAGAAGGTAATGGGTGGATTTTGTCACACTGTCTTACAACAACAGCCATGTGG
CTGAGTCCTTTCTGGAATTCGTTCAATATGTCATATAAAGGCAAGATTTCTGGAACTCTA
AAACAAAGCAAGAAATCAGCTTTTGAAGCACATTCAGTGGGGCTTAGTTACTTAGATTTG
GAAGTTTCACCTTGGAATCCTTCTTATCGTGACATTCTTGACAGGTATTTCTTCATCTGG
GTTGATAGTTAATAAGTTCTTCACCTGAAGATAATAGATATGAATGGTTAGAAGTGGAAC
CATAACACTAAAGAGGAAAATATTTGTGAAAAATGACTTCACAGAGAAGCGAGTCAAATT
GTGGAAGTAATGCATGAGACTGGGAGTTACCAGAACAAGGCCGATNATGTTAGTTGGCTG
CTCATAGTATACTGGAACTCTGCTATGCCCTTTCT
   
Sequence Name 14-K114131-SALK_042206C-66kh
GenBank Accession LN484672 [GenBank]
Sequencing primer gene specific primer, 66kh: 5'-ACTTGATTCTTGAGAAAGGGCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114131-SALK_042206C-66kh
ATCGGCCTTGTTCTGGTAACTCCCAGTCTCATGCATTACTTCCACAATTTGACTCGCTTC
TCTGTGAAGTCATTTTTCACAAATATTTTCCTCTTTAGTGTTATGGTTCCACTTCTAACC
ATTCATATCTATTATCTTCAGGTGAAGAACTTATTAACTATCAACCCAGATGAAGAAATA
CCTGTCAAGAATGTCACGATAAGAAGGATTCCAAGGTGAAACTTCCAAATCTAAGTAACT
AAGCCCCACTGAATGTGCTTCAAAAGCTGATTTCTTGCTTTGTTTTAGAGTTCCAGAAAT
CTTGCCTTTATATGACATATTGAACGAATTCCAGAAAGGACTCAGCCACATGGCTGTTGT
TGTAAGACAGTGTGACAAAATCCACCCATTACCTTCTAAAAATGGTAAACTGCTTCATTA
ATCGACAAAACAATGTCTTGCTGAGTTACAAGTATTGAGAATCTTTGCTTTGGTTTCTAA
TAGGGAGTGTTAAGGAAGCCCGAGTGGATGTGGATAGTGAGGGAACTCCTACTCCTCAGG
AGAGAATGTTAAGGACAAAAAGATCGCTTCAAAAGTGGAAGAGCTTTCCGAATCGAGCAA
GTTCGTTTAAAGGAGGGTCAAAGAGCAAGAAGTGGTCAAAAGACAATGATGCAGACATCC
TGCAATTGAATGGCAATCCGCTGCCTAAACTTGCTGAGGAAGAAGAAGCAGTCGGAATCA
TTACGATGGAGGATGTCATTGAAGAACTTCTGCAGGTAAGGAATCTTATATAAGTTAAAA
ACAAAAATTCTTTTTCACGGATTAATTATTGCTTGGCTTGTAAACAAATTGACGCTNAGA
CNACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT

Last Updated on 06.12.2018 16:11