Sequences from confirmation amplicon


Confirmation sequences for line SALK_042400 and hit At1g77330 are shown.
Line ID SALK_042400
Locus At1g77330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29063055
Primers used for PCR BI34 - R204, show primer details
   
Sequence Name 75-K106742-SALK_042400C-R210
GenBank Accession LN486123 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K106742-SALK_042400C-R210
CATGCATCACATGGCAAAACAAAAAAAAACAAAAAAATATAAAGCCTGGAGACCATATGC
CAAATTTTTAACTATATAATTTGGCATTTATAGCTCAAGAGAAAATACATAATGGGTGTA
GATAAAACAGTGATAGTACTGTAAAAATAAATAAAATAGTACATTTAGAGAGACTTTACA
GCTAGAAAACGAGGCTCTTTAGGCATGAACTTCTGGTTTGCATAAACATCCATGTAATCT
CCAAACACAAACTTTGGATACTTCTTCTCACTTCCTTCCTCTTCCGCCACCGCGGCTGGC
CCTATCGCCGCCTTGTACGACGGATTGTAGAAGGAAGCTATAGACCTTCTGTTTCCTTCC
TCCCTCGCCAACACCCTGTGCCACGCACTCTTGTACCTGCCAACCACATTTTGGTTAAAA
CTTTATACTCAAAATTGTATATTTTGTACTAGTGTTGTCGTCGTGTCACCACTGTTTCCG
GATCAAGTCGAGATTAGTGTTTTGGTTCGTAAAAGATGACTGAAAAATACCTTCCGTTGC
TAAGAACTTCAATCTGATCACCAGTG
   
Sequence Name 91-K106743-SALK_042400C-BI34
GenBank Accession LN486339 [GenBank]
Sequencing primer gene specific primer, BI34: 5'-CAACACTGGTGATCAGATTGAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K106743-SALK_042400C-BI34
TCTTTACGAACCAAACACTAATCTCGACTTGATCCGGAAACAGTGGTGACACGACGACAA
CACTAGTACAAAATATACAATTTTGAGTATAAAGTTTTAACCAAAATGTGGTTGGCAGGT
ACAAGAGTGCGTGGCACAGGGTGTTGGCGAGGGAGGAAGGAAACAGAAGGTCTATAGCTT
CCTTCTACAATCCGTCGTACAAGGCGGCGATAGGGCCAGCCGCGGTGGCGGAAGAGGAAG
GAAGTGAGAAGAAGTATCCAAAGTTTGTGTTTGGAGATTACATGGATGTTTATGCAAACC
AGAAGTTCATGCCTAAAGAGCCTCGTTTTCTAACTGTAAAGTCACTCTAAATGTACTATT
TTATTTATTTTTACAGTACTATCACTGTTTTATCCACACTCACTATGTATTTTCTCTTAC
GCCGCAAATGCCCAATTATATATATTAAAAAATTGTTATCCCCTG

Last Updated on 06.12.2018 16:11