Sequences from confirmation amplicon


Confirmation sequences for line SALK_042723 and hit At4g02350 are shown.
Line ID SALK_042723
Locus At4g02350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1038674
Primers used for PCR 14rb - LBb3, show primer details
   
Sequence Name 71-K116055-SALK_042723C-14rb
GenBank Accession LN486038 [GenBank]
Sequencing primer gene specific primer, 14rb: 5'-AATCGGAGATAAAACAATGCAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K116055-SALK_042723C-14rb
GGCTCACACCGCCGGAGCCGGTATGATTCGGCGGAGAAGCTCGACGAGCTCTTGATATCG
TCTGCCATTTGTAACGGTGAAGATTTAGGACCATTTGTTCGGAAAACTTTCGGTACAGGG
AAACCAGAGACTCTTCTTCACCATCTCAAGTTTTTCGCACGATCTAAGGAATCAGAGATC
GAAGAGGTATGTAAAGCTCACTATCAAGACTTTATCCACGCCGTAGACGATCTTAAATCG
CTTCTCTCAGATGTTGAATCGCTTAAATCTGCGCTTTCCGATTCAAACTCTAAACTCCAA
TCTGTTGCGGCGCCGCTTCTTTCGTCTCTCGATTCGCTTGTTGAGGCTCAGACTGTGTCT
AAGAACGTCGATCTGGCGATTGGAGCGGTGACTCATTGTGTTCGTGTTATGGAACTTGTC
TCTCGAGCTAATCAGCATCTTCAGAGCGGTAATTTCTACATGGCGTTGAAATGTGTGGAC
TCGATTGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTG
TTTGATGGTGGTTCCG
   
Sequence Name 44-K116054-SALK_042723C-R210
GenBank Accession LN485376 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116054-SALK_042723C-R210
TTTGTCATCGAGTCCCACATTTCAACGCCATGTAGAAATTACCGCTCTGAAGATGCTGAT
TAGCTCGAGAGACAAGTTCCATAACACGAACACAATGAGTCACCGCTCCAATCGCCAGAT
CGACGTTCTTAGACACAGTCTGAGCCTCAACAAGCGAATCGAGAGACGAAAGAAGCGGCG
CCGCAACAGATTGGAGTTTAGAGTTTGAATCGGAAAGCGCAGATTTAAGCGATTCAACAT
CTGAGAGAAGCGATTTAAGATCGTCTACGGCGTGGATAAAGTCTTGATAGTGAGCTTTAC
ATACCTCTTCGATCTCTGATTCCTTAGATCGTGCGAAAAACTTGAGATGGTGAAGAAGAG
TCTCTGGTTTCCCTGTACCGAAAGTTTTCCGAACAAATGGTCCTAAATCTTCACCGTTAC
AAATGGCAGACGATATCAAGAGCTCGTCGAGCTTCTCCGCCGAATCTATACCGGCTCCGG
CGGTTGTGGAGCCAACTTTCCGCCGTCCTTTCGACGATTGCATTGTTTTATCTCCAAT

Last Updated on 06.12.2018 16:11