Sequences from confirmation amplicon


Confirmation sequences for line SALK_042866c and hit At1g06660 are shown.
Line ID SALK_042866c
Locus At1g06660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2039760
Primers used for PCR 18ty - LBb3, show primer details
   
Sequence Name 30-K117180-SALK_042866C-18ty
GenBank Accession LN485136 [GenBank]
Sequencing primer gene specific primer, 18ty: 5'-GAAAAATTTCAGAGCTTCCACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117180-SALK_042866C-18ty
ATTCTATAAGAAAGTCATTAATGTAAGGAAACACATTTACACAGGGATCCATGCATTCAG
ATATATAGACATCCGTGATAGAAATATGAACAGTGAATTAAGAGCAGAGGGAAAAAAGAA
GAACAAAACTTCATTATATCAACAAACCTAAGACGACCTCAGTAACAAAAAACATAGAAC
TCATCTTCTCATTACCTGAAGAAGAGATGTGGTAATTATAAGACTCGCAAAAACAAAATC
AGGGTTTCAAAAACAAGAAATTGATTCATGTGGAGTTAAGTTGAGATATCAAAATCAATC
CAAGGGCACAAGTCTTGATCATGATAAAAGGAATAAGAATCAGTATGGTTAGAATCACAT
GAATAGAGGCAATGCTTCAAATAAAAATCATACCTCATCCCTAAGGCCTTTGTCAATATG
TATAGAATCTAAATCAAATCTTTCTTTGTCAAGGCAAGGCGAAGAAGCTGATTTCTCTAT
AAAAATCAAGAACAAAACCCTTATATTATTAACAAGAACAGTACCAAAAACAAGTAATCA
TCTCGATTCCTGATATAAACACATCGAAAAAGAGATTTACCTTCAGAAAGAAACAAAGCT
GACAAATCATTCTGCGAGTCCTGTGCTTTCTGAAGATAAATAAGAAAAGAAACAAATCAA
GCGGACGTTTTGTACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 09-K117179-SALK_042866C-R210
GenBank Accession LN484531 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117179-SALK_042866C-R210
CACAATATATCCTGTACAAACGTCCGCTTGATTTGTTTCTTTTCTTATTTATCTTCAGAA
AGCACAGGACTCGCAGAATGATTTGTCAGCTTTGTTTCTTTCTGAAGGTAAATCTCTTTT
TCGATGTGTTTATATCAGGAATCGAGATGATTACTTGTTTTTGGTACTGTTCTTGTTAAT
AATATAAGGGTTTTGTTCTTGATTTTTATAGAGAAATCAGCTTCTTCGCCTTGCCTTGAC
AAAGAAAGATTTGATTTAGATTCTATACATATTGACAAAGGCCTTAGGGATGAGGTATGA
TTTTTATTTGAAGCATTGCCTCTATTCATGTGATTCTAACCATACTGATTCTTATTCCTT
TTATCATGATCAAGACTTGTGCCCTTGGATTGATTTTGATATCTCAACTTAACTCCACAT
GAATCAATTTCTTGTTTTTGAAACCCTGATTTTGTTTTTGCGAGTCTTATAATTACCACA
TCTCTTCTTCAGGTAATGAGAAGATGAGTTCTATGTTTTTTGTTACTGAGGTCGTCTTAG
GTTTGTTGATATAATGAAGTTTTGTTCTTCTTTTTTCCCTCTGCTCTTAATTCACTGTTC
ATATTTCTATCACGGATGTCTATATATCTGAATGCATGGATCCCTGTGTAAATGTGTTTC
CTTACATTAATGACTTTCTTATTAGAATGCAGGGATTCTTTTTCAGAAACTCTGTAATTT
TGTTGTGGAAGCTCTAAAAATT

Last Updated on 06.12.2018 16:11