Sequences from confirmation amplicon


Confirmation sequences for line SALK_043673 and hit At1g73230 are shown.
Line ID SALK_043673
Locus At1g73230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27541104
Primers used for PCR BI24 - R204, show primer details
   
Sequence Name 67-K106742-SALK_043673C-R210
GenBank Accession LN485918 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K106742-SALK_043673C-R210
GTCATTTGTTACATTTTAAGGATGATGTAGTCATTCAGTTCATTAACCCTACAGGTAAAA
CATCAAATTTTATGGCTTTATAAATCTCAGGTTTCGTATAAAATTTATTGTATTAATGGT
TGTTGTGTCTCTGTTTTAATGTTGCTATCACAGTTCAAGCTTCAATTGCTGCTAACACAT
GGGTTGTGAGTGGTACACCACAGACGAAAAGTAAGTGTTTGTATCCTCATTGTTGATCCA
ATACTATTCTCGGTTTATGCATAGTCTAATCTCGGAATGTGAATTATCCTGCAGAATTGC
AAGACATTCTTCCTCAGATTATCAGCCAACTTGGTATGCAGCATTAAGTGTTACACTCTG
ATGTCCTTTCCCAAACCAGTGGTCCCTTAAGTTAACATTTTGTTTTCTTGCTTTGATAAT
CGCAGGACCAGATAACTTGGACAACCTGAAGAAGCTAGCAGAGCAATTCCAGAAACAAGC
TCCAGGTGCAGGTGATGTCCCAGCAACAATCCAAGAAGAGGACGATGATGATGATGTCCC
AGATCTTGTAGTGGGAGAGACTTTCGAGACCCCTGCTACTGAAGAGGCTCCCAAAGCTGC
TGCTTCTTAAAGGAGGAGGAAGAA
   
Sequence Name 83-K106743-SALK_043673C-BI24
GenBank Accession LN486253 [GenBank]
Sequencing primer gene specific primer, BI24: 5'-CTTCTTCCTCCTCCTCTAAGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K106743-SALK_043673C-BI24
TCTCTCCACTACAAGATCTGGGACATCATCATCATCGTCCTCTTCTTGGATTGTTGCTGG
GACATCACCTGCACCTGGAGCTTGTTTCTGGAATTGCTCTGCTAGCTTCTTCAGGTTGTC
CAAGTTATCTGGTCCTGCGATTATCAAAGCAAGAAAACAAAATGTTAACTTAAGGGACCA
CTGGTTTGGGAAAGGACATCAGAGTGTAACACTTAATGCTGCATACCAAGTTGGCTGATA
ATCTGAGGAAGAATGTCTTGCAATTCTGCAGGATAATTCACATTCCGAGATTAGACTATG
CATAAACCGAGAATAGTATTGGATCAACAATGAGGATACAAACACTTACTTTTCGTCTGT
GGTGTACCACTCACAACCCATGTGTTAGCAGCAATTGAAGCTTGAACTGTGATAGCAACA
TTAAAACAGAGACACAACAACCATTAATACAATAAATTTTATACGAAACCTGAGATTTAT
AAAGCCATAAAATTTGATGTTTTACCTTTAGGGTTAATGAACTGAATGACTACATCATCC
TTAAAAATGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTT
Primers used for PCR 71xo - LBb3, show primer details
   
Sequence Name 69-K119298-SALK_043673C-71xo
GenBank Accession LN485993 [GenBank]
Sequencing primer gene specific primer, 71xo: 5'-GAAATCGATTAACAAGGTAAGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K119298-SALK_043673C-71xo
TATTTCCAGATATTCGATTGTTTCCTTCTGATTTAGCACAAGGGTCAAGATCATTTTTTT
GTTTTTGTTCTTCCTCGATTCATATGTTTTTTTTCATTGATTACTGTTTTTGATATTGGT
TATCTTGTTTTCCAATAACTTGTTAGTTAGTTAAAAGTAATGTTTGTGTAATGGTGACCT
AATCGATTGAAAAAATTGAAACTTTAGGTTAAGCTCATTGTAACAATACGAGCTTTGTTC
TTCTCGCTGCACTGTTCTCATAATCCTAGTGTAATCAATTTGTGGGTTGTGACTATTGCT
CAGTATTACTGTTTTTGTCATTGGCTAATTAATAACAGTATTAGTTATTGTATATGCTTA
ATTTATAAGATACATTTTGGGCTAAATGGGTTTAGCTTTGTGTGTTTGTTTTGAAAAACA
GATGAATAGGGAAAAGTTGATGAAGATGGCTAACACTGTCCGCACTGGCGGAAAGGGGAC
AGTAAGAAGGTATACTCATTTATGTCTTTTCTGTGAATTTGTGTTTTTGCCATTTTTCAT
GTTATATTCTCTAAACATTTTGACTCTCATTTCAGAAAGAAGAAGGCTGTTCACAAGACC
ACTACAACCGATGACAAGAGGCTCCAGAGCACTCTTAAGAGAGTTGGAGTCAATTCCATT
CCCGCCATTGAAGAAGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTG
   
Sequence Name 21-K119297-SALK_043673C-R210
GenBank Accession LN484902 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K119297-SALK_043673C-R210
GTCATTTGTTACTTCTTCATGGCGGGAATGGAATTGACTCCAACTCTCTTAAGAGTGCTC
TGGAGCCTCTTGTCATCGGTTGTAGTGGTCTTGTGAACAGCCTTCTTCTTTCTGAAATGA
GAGTCAAAATGTTTAGAGAATATAACATGAAAAATGGCAAAAACACAAATTCACAGAAAA
GACATAAATGAGTATACCTTCTTACTGTCCCCTTTCCGCCAGTGCGGACAGTGTTAGCCA
TCTTCATCAACTTTTCCCTATTCATCTGTTTTTCAAAACAAACACACAAAGCTAAACCCA
TTTAGCCCAAAATGTATCTTATAAATTAAGCATATACAATAACTAATACTGTTATTAATT
AGCCAATGACAAAAACAGTAATACTGAGCAATAGTCACAACCCACAAATTGATTACACTA
GGATTATGAGAACAGTGCAGCGAGAAGAACAAAGCTCGTATTGTTACAATGAGCTTAACC
TAAAGTTTCAATTTTTTCAATCGATTAGGTCACCATTACACAAACATTACTTTTAACTAA
CTAACAAGTTATTGGAAAACAAGATAACCAATATCAAAAACAGTAATCAATGAAAAAAAA
CATATGAATCGAGGAAGAACAAAAACAAAAAAATGATCTTGACCCTTGTGCTAAATCAGA
AGGAAACAATCGAATATCTTGGAAATGAAATGAGTGAACGATTTGCTGAAGTGGGCTTAC
CTTGTTATTCGATTT

Last Updated on 06.12.2018 16:11