Sequences from confirmation amplicon


Confirmation sequences for line SALK_043724c and hit At1g54360 are shown.
Line ID SALK_043724c
Locus At1g54360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20292317
Primers used for PCR 38wu - LBb3, show primer details
   
Sequence Name 34-K118859-SALK_043724C-38wu
GenBank Accession LN485211 [GenBank]
Sequencing primer gene specific primer, 38wu: 5'-AATTATCCAATCTTGTTGGCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118859-SALK_043724C-38wu
ATATTGAGCCATATGTAAGTTTTGTGCGCACACTTTTTTTTTGTTTTTTTTTGTTTCTCT
TCTCCCTTCTCCTCCTTCTGTTGACTTTTAACTTGTTGTATTCTGTTCAGCTGCATCAGT
TGATGCCGTCTATTATCACTTGCCTTATTGCCAAGCGCTTAGGAAGAAGGTCATCTGATA
ATCACTGGGACTTGAGAAACTTCACTGCCAGCACAGTTGCTTCAACGTGCAAAAGGTTAA
TGTTACATTCTTGCTTTGTGATTTCATGCATCTTATACAACCATATAACTTGCAATCACA
TTAGTGAACTTTTTACAGGTTTGGACACGTTTATCACAATTTGTTGCCACGTGTCACAAG
ATCACTGCTTCATACCTTTTTGGACCCAACCAAGGCACTCCCACAACACTATGGGGCAAT
TCAAGGGATGGTAGCCCTTGGGCTTAATATGGTACTTCCTCTTGATGCTTCTTGATTATC
ATAACGAAAAGTAGCACTTTAGGTTGTGCGTCTAGAATTTTTACATAAGAAAGCATAAGT
TTAAAACTTAATAAACAAATTGACGCTAAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCGGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGATGGTTCCAA
   
Sequence Name 10-K118858-SALK_043724C-R210
GenBank Accession LN484570 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118858-SALK_043724C-R210
GTCATTTGTTTATAAGTTTTAACTTATGCTTTCTTATGTAAAAATTCTAGACGCACAACC
TAAAGTGCTACTCTTCGTTATGATAATCAAGAAGCATCAAGAGGAAGTACCATATTAAGC
CCAAGGGCTACCATCCCTTGAATTGCCCCATAGTGTTGTGGGAGTGCCTTGGTTGGGTCC
AAAAAGGTATGAAGCAGTGATCTTGTGACACGTGGCAACAAATTGTGATAAACGTGTCCA
AACCTGTAAAAAGTTCACTAATGTGATTGCAAGTTATATGGTTGTATAAGATGCATGAAA
TCACAAAGCAAGAATGTAACATTAACCTTTTGCACGTTGAAGCAACTGTGCTGGCAGTGA
AGTTTCTCAAGTCCCAGTGATTATCAGATGACCTTCTTCCTAAGCGCTTGGCAATAAGGC
AAGTGATAATAGACGGCATCAACTGATGCAGCTGAACAGAATACAACAAGTTAAAAGTCA
ACAGAAGGAGGAGAAGGGAGAAGAGAAACAAAAAAAAACAAAAAAAAAGTGTGCGCACAA
AACTTACATATGGCTCAATATGTACATGAGGGTTATGAAGGAGGCTTCTAGCAAGACGCA
TCAAAGCCAACAAATGGGGATAA

Last Updated on 06.12.2018 16:11