Sequences from confirmation amplicon


Confirmation sequences for line SALK_044034c and hit At5g66490 are shown.
Line ID SALK_044034c
Locus At5g66490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26547971
Primers used for PCR 08uk - LBb3, show primer details
   
Sequence Name 63-K118240-SALK_044034C-08uk
GenBank Accession LN485832 [GenBank]
Sequencing primer gene specific primer, 08uk: 5'-TGACATACTCCAAGTACCTAGCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118240-SALK_044034C-08uk
TCTTCTCCTCTTAGACTCATTTCGAAGAACTAAATAGAGGGTGAGCCGAACCCCTGGTTT
TCTCCACCGGTTTATTCACCTTCGGGGATTGACCATGGGGATTTGTAGGAACGCTCTCCG
GTTTCACTTCTAAAAGCCCTGCGCCTGCTTCTTCTTCGTCGCCGAGCTTCTTGTAGCTGC
CTCGAACCTTCCTCGTGCTCAGCGCTCTAATCATTATTTCTTGTGGTTTTGATCAATTCT
CTCTGGTGATATGTTTTTAAGAAAGGATAGATTTTGAAAAGGACACATCTTTTGACTTCT
CTTCTCCTTATCCCAACGACTCCTTTAACTTAAACCTTATGTGACATTGCCCTTTCACCT
ACTACTATTCGTGGGTCAATGCTACTTTATTTTTGACTATGTCTTCTTTGGTGTTTGACT
TAATTAGGACAACTTAAGAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTCCGAATCCGGCAAAA
   
Sequence Name 40-K118239-SALK_044034C-R210
GenBank Accession LN485302 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118239-SALK_044034C-R210
CATTTCTTAGTTGTCCTAATTAANTCAAACACCAAAGAAGACATAGTCAAAAATAAAGTA
GCATTGACCCACGAATAGTAGTAGGTGAAAGGGCAATGTCACATAAGGTTTAAGTTAAAG
GAGTCGTTGGGATAAGGAGAAGAGAAGTCAAAAGATGTGTCCTTTTCAAAATCTATCCTT
TCTTAAAAACATATCACCAGAGAGAATTGATCAAAACCACAAGAAATAATGATTAGAGCG
CTGAGCACGAGGAAGGTTCGAGGCAGCTACAAGAAGCTCGGCGACGAAGAAGAAGCAGGC
GCAGGGCTTTTAGAAGTGAAACCGGAGAGCGTTCCTACAAATCCCCATGGTCAATCCCCG
AAGGTGAATAAACCGGTGGAGAAAACCAGGGGTTCGGCTCACCCTCTATTTAGTTTCTTC
GAAATGAGTCTAAAGAGGAAGAAGAAGAAGAAGAGCACGACGACTGCAAAACCAGAATTC
GCTAGGTACTTGAGGTATGTC

Last Updated on 06.12.2018 16:11