Sequences from confirmation amplicon


Confirmation sequences for line SALK_044115c and hit At1g71100 are shown.
Line ID SALK_044115c
Locus At1g71100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26815582
Primers used for PCR 20eb - LBb3, show primer details
   
Sequence Name 51-K110195-SALK_044115C-20eb
GenBank Accession LN485531 [GenBank]
Sequencing primer gene specific primer, 20eb: 5'-GTCTCTCACAGATTTTGTGCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K110195-SALK_044115C-20eb
GGTTCTGATTCGATCCCCTTGTCCGGCCACTGAGGATCTCGCCGCCGTCAACTCGGCGCC
GCCACTCTCAAACCTCACGCAAGAAGAACTCAAGAAAATCGCCGCTTATAAAGCCGTCGA
ATTCGTCGAATCTGGAATGGTTATCGGTCTCGGCACTGGCTCCACCGCGAAACACGCCGT
GGCTCGTATCAGCGAGCTTCTCCGAGAAGGAAAACTCAAGGATATAATCGGAATCCCTAC
TTCAACCACAACGCATGAGCAAGCGGTGTCTCTAGGGATACCATTATCCGATCTAGATTC
GCATCCGGTGGTGGATCTATCAATCGATGGCGCCGACGAAGTCGATCCGGCTCTTAATCT
GGTGAAAGGACGCGGCGGATCGTTATTGAGAGAGAAGATGATTGAAGGAGCATCTAAGAA
GTTCGTTGTGATTGTTGATGAATCGAAGCTTGTCAAGTATATCGGAGGAAGCGGACTCGC
TGTTCCGGTGGAGGTTGTTCCGTTCTGCTGCGATTTCACAAGGGGGAAACTGGAAGAGCT
ATTTAGAGATTCGGGATGTGTTGCGAAGCTGAGGATGAAAATCGGATCTAACGGCGAAGA
GGCGGCGCCGGCTGTGACGGATAATAGGAATTATGTGGTGGATTTGTATTTGGAGAGAGA
TATTGG
   
Sequence Name 43-K110194-SALK_044115C-R210
GenBank Accession LN485350 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K110194-SALK_044115C-R210
GCGTCATTTGTTTACCACAATATATCCTGATAGTTTGTCTAACCACCATCGAAAATCAAA
ATCAAACCATAAACCCTAATGGTTTTCAAATGACACTTTTATCCAAACCTATCCTTGACG
GTAACACCAAATTTGCCGGCGACGATCAACGTAGTCGCCATTCCCAGAAACATCCCATGC
TCCACTACTCCAGGAAACCTCAATATCGCCTCACTCGCCACCTCTAAATCTCCAATATCT
CTCTCCAAATACAAATCCACCACATAATTCCTATTATCCGTCACAGCCGGCGCCGCCTCT
TCGCCGTTAGATCCGATTTTCATCCTCAGCTTCGCAACACATCCCGAATCTCTAAATAGC
TCTTCCAGTTTCCCCCTTGTGAAATCGCAGCAGAACGGAACAACCTCCACCGGAACAGCG
AGTCCGCTTCCTCCGATATACTTGACAAGCTTCGATTCATCAACAATCACAACGAACTTC
TTAGATGCTCCTTCAATCATCTTCTCTCTCAATAACGATCCGCCGCGTCCTTTCACCAGA
TTAAGAGCCGGATCGACTTCGTCGGCGCCATCGATTGATAGATCCACCACCGGATGCGAA
TCTAGATCGGATAATGGTATCCCTAGAGACACCGCTTGCTCATGCGTTGTGGTTGAAGTA
GGGATTCCGATTATATCCTTGAGTTTTCCTTCTCGGAGAAGCTCGCTGATACGAGCCACG
GCGTGTTTCGCGGTGGAGCCAGTGCCGAGACCGA

Last Updated on 06.12.2018 16:11