Sequences from confirmation amplicon


Confirmation sequences for line SALK_044381c and hit At5g07340 are shown.
Line ID SALK_044381c
Locus At5g07340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2318075
Primers used for PCR 21eb - LBb3, show primer details
   
Sequence Name 49-K110195-SALK_044381C-21eb
GenBank Accession LN485476 [GenBank]
Sequencing primer gene specific primer, 21eb: 5'-TCAAAATGACGTTCGTAGGTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K110195-SALK_044381C-21eb
GATATCTCTCTCTCTGATCACTTAGTACTTACTTAGTAGTTACTTCTCTGTCTTATTTGA
TTTTGAGTCTCTGTTTTGTCTCTTCTCTTGAGATTGCGATAATGAGAGAACGGATTATTA
CATTCGTTTCGCTGCTTCTTGTAGCTTTGCTTTCGTTTCCCAGTGTTAGCTACTGTGACG
ACCAAACGGTGATTGGTCACTTCCTTGAAAAAGTTCCTATTTTAAAGATTTGTTCATCGA
ATCTCCAGTAACATGGTGTTTTTGTTGTTGTTCAGATCCTGTATGAATCGTTTGACGAGC
CTTTTGATGGTCGCTGGGTCGTTTCAGAGAAAGCTGAATACCAAGGTAAAGCCATGTCGT
CGTAGTGAATTCGTGTTTTGAATTTTTTGGTTTTCTTTCGTCTGTTGAAGTAAAAACAAG
TTAGAATTAGTGTAATGGAAGTTACAATCAAAAGGGGGTCTTAAAGTTTTGGTTTTTATT
GGAACAGATTTAGAAATGTTTACTTTTACTATAAGTGGATAAGAAACAGACTTTGTGTGA
GCTCTTAAAAGGTTGCTTCTTATTTGAAATCTTTTAGGTGTGTGGAAGCACGAGAAGAGT
GAAGGGCATGATGATTATGGACTTCTAGTGAGTGAGAAAGCTAAGAAGTACGGAATAGTT
AAAGAGCTTGATGTTGATGAGCCTCTAAACCTTAACGAAGGAACTGTTGTTCTTCAATAC
GAGGCTCGTTTCCAAGAAGGGCTTGAGTGTGGTGGTGCTTACTTAAAGTATCTTCGTCCT
CAAGAAGCTGGATGGGTTCCTCAAGGGTTTGATAATGATTCTCCTTACTCTATCATGTTT
GGACCTGATAAGTGTGGTGCTACCAACAAGGTTCATTTGGACAAATTGAC
   
Sequence Name 41-K110194-SALK_044381C-R210
GenBank Accession LN485308 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K110194-SALK_044381C-R210
GCGTCATTTGTCCAATGAACCTTGTTGGTAGCACCACACTTATCAGGTCCAACATGATAG
AGTAAGGAGAATCATTATCAAACCCTTGAGGAACCCATCCAGCTTCTTGAGGACGAAGAT
ACTTTAAGTAAGCACCACCACACTCAAGCCCTTCTTGGAAACGAGCCTCGTATTGAAGAA
CAACAGTTCCTTCGTTAAGGTTTAGAGGCTCATCAACATCAAGCTCTTTAACTATTCCGT
ACTTCTTAGCTTTCTCACTCACTAGAAGTCCATAATCATCATGCCCTTCACTCTTCTCGT
GCTTCCACACACCTAAAAGATTTCAAATAAGAAGCAACCTTTTAAGAGCTCACACAAAGT
CTGTTTCTTATCCACTTATAGTAAAAGTAAACATTTCTAAATCTGTTCCAATAAAAACCA
AAACTTTAAGACCCCCTTTTGATTGTAACTTCCATTACACTAATTCTAACTTGTTTTTAC
TTCAACAGACGAAAGAAAACCAAAAAATTCAAAACACGAATTCACTACGACGACATGGCT
TTACCTTGGTATTCAGCTTTCTCTGAAACGACCCAGCGACCATCAAAAGGCTCGTCAAAC
GATTCATACAGGATCTGAACAACAACAAAAACACCATGTTACTGGAGATTCGATGAACAA
ATCTTTAAAATAGGAACTTTTTCAAGGAAGTGACCAATCACCGTTTGGTCGTCACAGTAG
CTAACACTGGGAAACGAAAGCAAAGCTACCAGAAGCAGCGAAACGAAAGTAATAATCCGT
TCTCTCATTATCGCAATCTCAAGAGAAGA

Last Updated on 06.12.2018 16:11