Sequences from confirmation amplicon


Confirmation sequences for line SALK_044671c and hit At4g15640 are shown.
Line ID SALK_044671c
Locus At4g15640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8919426
Primers used for PCR 43kq - LBb3, show primer details
   
Sequence Name 54-K113870-SALK_044671C-43kq
GenBank Accession LN485608 [GenBank]
Sequencing primer gene specific primer, 43kq: 5'-TGTTATCAGGTGATACAGAGTCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113870-SALK_044671C-43kq
GTCACACACTCTTTCTACTGCCTTTTCTTGTGCAAAGGTATTAACACATCTCTTTTTCGG
TCTCATGTAATAACCAAATACCGAATTATATTTATAAATATTGCTCTTTTCACAGGGTTT
CCTACTAGAAAGTAAACCTGAAGAAGCTGCTGCCGTCATCCAGATTATCTGCCAAGTGAG
CCCAAAACCTACCTTAATATCTCTTCTCTTCTGATACAACCTTTCTTTTAGCTTTGATAA
GTATTTAGTTTAAATATATGTGTATGACTGACTACGCAGGCATATCCTGATGAGAAAAAG
TCTGCAATTTCGACTGAATTTGAGAAACTTGTAAACGAGTGGCCTGTGGACGTTATCAAG
CACCAAACCGACGAAGATAAGAAGGTTAAAAGATATTCTCTCTATTTGTTATGACGCTNA
GACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACC
AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAG
CGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 62-K113869-SALK_044671C-R210
GenBank Accession LN485795 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K113869-SALK_044671C-R210
GTCTACAATAGAGAGAATATCTTTTAACCTTCTTATCTTCGTCGGTTTGGTGCTTGATAA
CGTCCACAGGCCACTCGTTTACAAGTTTCTCAAATTCAGTCGAAATTGCAGACTTTTTCT
CATCAGGATATGCCTGCGTAGTCAGTCATACACATATATTTAAACTAAATACTTATCAAA
GCTAAAAGAAAGGTTGTATCAGAAGAGAAGAGATATTAAGGTAGGTTTTGGGCTCACTTG
GCAGATAATCTGGATGACGGCAGCAGCTTCTTCAGGTTTACTTTCTAGTAGGAAACCCTG
TGAAAAGAGCAATATTTATAAATATAATTCGGTATTTGGTTATTACATGAGACCGAAAAA
GAGATGTGTTAATACCTTTGCACAAGAAAAGGCAGTAGAAAGAGTGTGTTGACTGTATGT
CTCTGATCTCTGTTTATCTACTTTCCATAGCGACTCTGTATCACTTGATAAC

Last Updated on 06.12.2018 16:11