Sequences from confirmation amplicon


Confirmation sequences for line SALK_044963c and hit At1g74910 are shown.
Line ID SALK_044963c
Locus At1g74910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28137136
Primers used for PCR 51dl - LBb3, show primer details
   
Sequence Name 62-K109940-SALK_044963C-51dl
GenBank Accession LN485788 [GenBank]
Sequencing primer gene specific primer, 51dl: 5'-GCTTCTTCTCACATCTGAGTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K109940-SALK_044963C-51dl
CTTACTGTGATGTTTGCTGCAGTTTCCCCTTGCCCAAATGCTCGGTATATTCTTTTTCAT
TAATCCAAACCTTGTGAGGTCTCATCTTATGGTTTTGAAGGTCGTTCTTAACTATTGCTA
CTTTTGATCAACAGAGGCTCACAGGGGATATGGTGGAATTGGAACTCTTCTTGTGATCAA
GGTTTGTGATTGTATATGATTAGTCTGGTTTTTGTTTTTTTGGCGGGTCAATCAAATTTG
CTTCTGCGTTTTGGTTTTTAGGTCTCTCCTGAATCAGCCAGCCAATTTGGAGAATTGGTT
GCAGATCCAGTTACTAATGAGCTGCTTCATTACACCGAGAAACCCGAAACTTTTGTATGT
ACATTTATTACTGAGATGACCCAATCTTTATAGCATCTTCAAGCTTTGATTCTTATGTTT
TATTTTGGAAACACAGGTCAGTGATCGAATTAACTGTGGTGTAAACAAATTGACGCTTAG
ACCACTTAATAACACATTGCGGACGTTTTTTATGTACTGGGGTGGTTTTTCTTTTCACCA
ATGAGACGGGCAACAGCTGATTGCCCTTCACCGCCCGGCCC
   
Sequence Name 53-K109939-SALK_044963C-R210
GenBank Accession LN485577 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K109939-SALK_044963C-R210
GTCATTTGTTACCCACANTTAATTCGATCACTGACCTGTGTTTCCAAAATAAAACATAAG
AATCAAAGCTTGAAGATGCTATAAAGATTGGGTCATCTCAGTAATAAATGTACATACAAA
AGTTTCGGGTTTCTCGGTGTAATGAAGCAGCTCATTAGTAACTGGATCTGCAACCAATTC
TCCAAATTGGCTGGCTGATTCAGGAGAGACCTAAAAACCAAAACGCAGAAGCAAATTTGA
TTGACCCGCCAAAAAAACAAAAACCAGACTAATCATATACAATCACAAACCTTGATCACA
AGAAGAGTTCCAATTCCACCATATCCCCTGTGAGCCTCTGTTGATCAAAAGTAGCAATAG
TTAAGAACGACCTTCAAAACCATAAGATGAGACCTCACAAGGTTTGGATTAATGAAAAAG
AATATACCGAGCATTTTGGGCAAGGGGAAACTGCAGCAAACATCACAGTTAAGCAGGAAG
ATATGAGACTGCAAGAAGT

Last Updated on 06.12.2018 16:11