Sequences from confirmation amplicon


Confirmation sequences for line SALK_045266 and hit At3g25585 are shown.
Line ID SALK_045266
Locus At3g25585 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9298101
Primers used for PCR 78by - LBb3, show primer details
   
Sequence Name 13-K108551-SALK_045266C-78by
GenBank Accession LN484633 [GenBank]
Sequencing primer gene specific primer, 78by: 5'-CATTTCCAGCAGTGAGAATCATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K108551-SALK_045266C-78by
AAGTTGGATCATAACGAGGTTTAATTAAAAAAAAAAATCTAGAACAATCCCATATTTTTT
TTTCTTAGAAACGTTACACAAAAAGGTTCTACATGAATTACCCTATTTCAACCGAACAAG
AAAAAAAACCCTAAAACAAAAACATTAATGAGTATACATCCAAAAGCATATCAGTCAATC
ATTTTTAATTCCAAGTAACAAAATATTCAAATTCGGGTTGGGATTTGCATTTTTTTTCTC
CCTGAAACAACATAACAAAAAAAGGTTAGAGCAAATAACCAAATAAACAAACACACTTGA
GATATGGAAGAAGAAAGCTAAAACCTTAAAAAGCATACCTTAAGATTTGCTTTTTCTTAT
AAATAAAAATTTCAATCACATTTTGTTTCTTCAAGCTTCTTTCCGAGTAATCCTGATGAA
AAAAAACAGATTAATCTTGTCAAATTTTGTAAACTTAAAACAATAAAATGGTAAGAAGAA
AGAGTATCTTAATCTTAATTCTAACCTGAAGCAGTAAATTCCAAGTGCAGTTGTGATCTC
ATGAATGACTGAAGTTGCAAAATGACGCTTAAACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGGGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCGGGCCCTGAAAGAGTTGCAGCAAGCGGCCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGGGGTTCCGAAATCGGCAAA
   
Sequence Name 03-K108550-SALK_045266C-R210
GenBank Accession LN484328 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K108550-SALK_045266C-R210
CGTATTTGCACTTCGTCATTCATGAGATCACAACTGCACTTGGAATCTACTGCTTCAGGT
TAGAATTAAGATTAAGATACTCTTTCTTCTTACCATTTTATTGTTTTAAGTTTACAAAAT
TTGACAAGATTAATCTGTTTTTTTTCATCAGGATTACTCGGAAAGAAGCTTGAAGAAACA
AAATGTGATTGAAATTTTTATTTATAAGAAAAAGCAAATCTTAAGGTATGCTTTTTAAGG
TTTTAGCTTTCTTCTTCCATATCTCAAGTGTGTTTGTTTATTTGGTTATTTGCTCTAACA
TTTTTTTGTTATGTTGTTTCAGGGAGAAAAAAAATGCAAATCCCAACACGAATCTGAATA
TTTTGTTACTTGGAATTAAAAATGATTGACTGATATGCTTTTGGATGTATACTCATTAAT
GTTTTTGTTTTAGTGTTTTTTTTCTTGTTCGGTTGAAATAGGGTAATTCATGTAGAACCT
TTTTGTGTAACGTTTCTAAGAAAAAAAAATATGGGATTGTTCTAGATTTTTTTTTTTAAT
TTAAACCTCGTTATTGATCCAACTTTTAGCTTTTTAAGCTTTATTTTATGATTCTCACTG
CTGGAAAG

Last Updated on 06.12.2018 16:11