Sequences from confirmation amplicon


Confirmation sequences for line SALK_045607c and hit At1g48605 are shown.
Line ID SALK_045607c
Locus At1g48605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17969744
Primers used for PCR 19ty - LBb3, show primer details
   
Sequence Name 31-K117180-SALK_045607C-19ty
GenBank Accession LN485153 [GenBank]
Sequencing primer gene specific primer, 19ty: 5'-TTCTGATTTGACAAAAGATGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117180-SALK_045607C-19ty
CTCTCTGAAATGAGAATCTCATAGATTGTTTGTATAAAATTTGATCCTGCTTCTGGATTC
AGATAGAGAAGAAGATGAATATGGAAGTGGATACAGTAACAAGGAAGCCTCGTATCTTAC
TAGCTGCAAGTGGAAGTGTGGCTTCAATTAAGTTCAGTAATCTCTGCCATTGTTTCTCAG
AATGGGCTGAAGTCAAAGCCGTCGCTTCAAAATCATCTCTCAATTTCGTTGATAAACCTT
CTCTACCTCAGAATGTGACTCTCTATACAGATGAAGATGAATGGTCTAGCTGGAACAAGA
TTGGTGATCCCGTTCTTCATATCGAGCTCAGACGCTGGGCTGATGTTATGATCATTGCTC
CTTTGTCTGCTAACACATTAGCCAAGGTACTAATAATCATAGGAAACGTAGCTGTAACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCG
   
Sequence Name 10-K117179-SALK_045607C-R210
GenBank Accession LN484564 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117179-SALK_045607C-R210
CTACGTTTCCTATGATTATTAGTACCTTGGCTAATGTGTTAGCAGACAAAGGAGCAATGA
TCATAACATCAGCCCAGCGTCTGAGCTCGATATGAAGAACGGGATCACCAATCTTGTTCC
AGCTAGACCATTCATCTTCATCTGTATAGAGAGTCACATTCTGAGGTAGAGAAGGTTTAT
CAACGAAATTGAGAGATGATTTTGAAGCGACGGCTTTGACTTCAGCCCATTCTGAGAAAC
AATGGCAGAGATTACTGAACTTAATTGAAGCCACACTTCCACTTGCAGCTAGTAAGATAC
GAGGCTTCCTTGTTACTGTATCCACTTCCATATTCATCTTCTTCTCTATCTGAATCCAGA
AGCAGGATCAAATTTTATACAAACAATCTATGAGATTCTCATTTCTCAGAGAGGAATTCA
GAATCCGAACATGGATTTTAACGATCATCTTTTGTCAAAATCAGAA

Last Updated on 06.12.2018 16:11