Sequences from confirmation amplicon


Confirmation sequences for line SALK_045660c and hit At1g25240 are shown.
Line ID SALK_045660c
Locus At1g25240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8845835
Primers used for PCR 34cv - LBb3, show primer details
   
Sequence Name 04-K109680-SALK_045660C-34cv
GenBank Accession LN484362 [GenBank]
Sequencing primer gene specific primer, 34cv: 5'-AACTAAACGAAAAACCGGTTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109680-SALK_045660C-34cv
AGGGGCAATATTAAACTTAAATAGAGAAACAATGTAAGCTTTGGTTTTAGAGGAAGAAAA
GAGAAGAAAAAGCGAAGAAGGAGGAGGAGGAGGAGGAGGCTGATGATGAAGCTGTGGAAA
CGGGCTTCCGGTGCTCTCAAAGACCGGAAAACCTTGTTTACCATCGGCTTCTCCCGGAAA
ACCTCCTTCCGCAACCCTGACCTTGATTCTGCCATCATCCACGCAACCTCTCACGATGAC
TCATCTGTCGATTACCACAATGCTCACCGTGTCTACAAATGGATCCGGTCCTCTCCCGCC
AATCTCAAGCCGCTTGTTCATGCTCTTTCATCTAGGGTTAACCGCACAAGAAGCTGGATT
GTAGCCTTGAAAGCCTTAATGCTTGTCCACGGTGTGCTTTGCTGCAAAGTCACGTCCCTT
CAAGAAATTCGTCGCCTCCCTTTCGATCTCTCGGATTTCTCGGATGGTCATTCCCGTCCC
AGCAAAACTTGGGGTTTCAACGCTTTTATCCGCGCCTATTTCTCGTTTCTTGACCAGTAC
TCTTTCTTTTTATCCGATCAAATCCGTCGTCGCCACAAAAAACCTCAGCTAGATTCGGTT
AACCAAGAGCTCGAAAGAATCGAGAAACTTCAGTCTCTTCTCCATATGCTTCTCCAGATC
CGTCCAATGGCTGACAACATGAAGAAGACGCTTATCCTTGAAGCCATGGAAGAATATATT
GTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTT
   
Sequence Name 20-K109679-SALK_045660C-R210
GenBank Accession LN484843 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109679-SALK_045660C-R210
GTCATTTGTTTACCCACAATATATTCTTCCATGGCTTCAGGATAAGCGTCTTCTTCATGT
TGTCAGCCATTGGACGGATCTGGAGAAGCATATGGAGAAGAGACTGAAGTTTCTCGATTC
TTTCGAGCTCTTGGTTAACCGAATCTAGCTGAGGTTTTTTGTGGCGACGACGGATTTGAT
CGGATAAAAAGAAAGAGTACTGGTCAAGAAACGAGAAATAGGCGCGGATAAAAGCGTTGA
AACCCCAAGTTTTGCTGGGACGGGAATGACCATCCGAGAAATCCGAGAGATCGAAAGGGA
GGCGACGAATTTCTTGAAGGGACGTGACTTTGCAGCAAAGCACACCGTGGACAAGCATTA
AGGCTTTCAAGGCTACAATCCAGCTTCTTGTGCGGTTAACCCTAGATGAAAGAGCATGAA
CAAGCGGCTTGAGATTGGCGGGAGAGGACCGGATCCATTTGTAGACACGGTGAGCATTGT
GGTAATCGACAGATGAGTCATCGTGAGAGGTTGCGTGGATGATGGCAGAATCAAGGTCAG
GGTTGCGGAAGGAGGTTTTCCGGGAGAAGCCGATGGTAAACAAGGTTTTCCGGTCTTTGA
GAGCACCGGAAGCCCGTTTCCACAGCTTCATCATCAGCCTCCTCCTCCTCCTCCTCCTTC
TTCGCTTTTTCCTCTCCTTTCCTCCTCCAAAACCAAAGCTTACATTGGTTTCTCTATTTT
AAGGTTTAATTATTTTGCCACCCTTGCTTTTGACCGGTGTAACCGGTTTT

Last Updated on 06.12.2018 16:11