Sequences from confirmation amplicon


Confirmation sequences for line SALK_046315c and hit At4g14150 are shown.
Line ID SALK_046315c
Locus At4g14150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8162132
Primers used for PCR 60fr - LBb3, show primer details
   
Sequence Name 24-K112129-SALK_046315C-60fr
GenBank Accession LN484975 [GenBank]
Sequencing primer gene specific primer, 60fr: 5'-GGAAACTGGACTCGTGATCTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112129-SALK_046315C-60fr
TCTCTGTCTCGTTCTCATATTATCCACGGTTTCTGGTTCATGGTTCTCAGTATGGCAACA
AGCATCCTCCATGGCAACATCTGAGTCCTCAGGAAGCCGCTTTTCTATAGATTGGCCATC
AGATGAATGTATACTCTTTACTCTGTTCATATCGTGATTGATCCCCTCAGAAGCTAAAGA
TGATTGCAAACCCACTTGAACACAAAGCCTTTCAACAGCAGCCTCATCAATTTCCATCTC
AATATCACCATCATTATCTTCATGAGGTAAGGACCTTGGATGACCCAGACCGAAGCTTCT
TAATAAATTCAAACTTCTACGTGCATTCCATGCAGTGGAATAAGCAACATTTGGATTGGT
TGGATTATTTCCATCATTCTTCATCCTTTGTAGTTCATCCTGCACAATTTATCCATTAAA
AGCCATATTCATATTTATATTTCTATCTNATAAAATATGTAAAAGAATCCGTACCCTCAG
CTGGTGTATCACTCCACGCAAGAAATTTACATCATCCTGCATCACTTCATTGACAACAGC
CTTATTCTGTATTGCCTTTGCACGCTGAGCAAATCTCAAGGTGCTGAAGGTTTCACTCCT
GCAACTGCAGTTTTCGTATATACTTTATATTCTAGAAGATGGAGATAAAATAATTGCTGA
AGTGGAAATAGGGGTCCTGCGTTACCTTTGAGAAGGAGAAACTGCACAAACCATTGCTAA
CTTTGCATTCCCACCAAGAGACTCTTGCAATAAAAATGTTAATCTGGAATCCCTGTAAGG
TATATGCCTTGGCTTCCCTGTTTGAGAAATCTCTGCGAGGATGTTAATCAAGTTCCTGCA
CGTAACANATTTATATGGTAAACATGTAGTTGATAATTTCATCTTGTGGTAAACATCCTG
CCAATAACAGGACTGGTGAACAAACCAAGCAAATCTNTTTCTTTTTACCAAGCAAAATTT
GTATATCCTCAGCAAAAAGGTGTAAACAAATTGACGCTNNAGACACTTAATACACATTGC
GAACGTTTTTATGTAC
   
Sequence Name 08-K112128-SALK_046315C-R210
GenBank Accession LN484491 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112128-SALK_046315C-R210
TTTGTTACCCCTTTTGCTGAGGATATACAAATTTTGCTTGGTAAAAAGAAAAAGATTTGC
TTGGTTTGTTCACCAGTCCTGTTATTGGCAGGATGTTTACCACAAGATGAAATTATCAAC
TACATGTTTACCATATAAATTTGTTACGTGCAGGAACTTGATTAACATCCTCGCAGAGAT
TTCTCAAACAGGGAAGCCAAGGCATATACCTTACAGGGATTCCAGATTAACATTTTTATT
GCAAGAGTCTCTTGGTGGGAATGCAAAGTTAGCAATGGTTTGTGCAGTTTCTCCTTCTCA
AAGGTAACGCAGGACCCCTATTTCCACTTCAGCAATTATTTTATCTCCATCTTCTAGAAT
ATAAAGTATATACGAAAACTGCAGTTGCAGGAGTGAAACCTTCAGCACCTTGAGATTTGC
TCAGCGTGCAAAGGCAATACAGAATAAGGCTGTTGTCAATGAAGTGATGCAGGATGATGT
AAATTTCTTGCGTGGAGTGATACACCAGCTGAGGGTACGGATTCTTTTACATATTTTATA
AGATAGAAATATAAATATGAATATGGCTTTTAATGGATAAATTGTGCAGGATGAACTACA
AAGGATGAAGAATGATGGAAATAATCCAACCAATCCAAATGTTGCTTATTCCACTGCATG
GAATGCACGTAGAAGTTTGAATTTATTAAGAAGCTTCGGTCTGGGTCATCCAAGGTCCTT
ACCTCATGAAGATAATGATGGTGATATTGAGATGGAAATTGATGAGGCTGCTGTTGAAAG
GCTTTGTGTTCAAGTGGGTTTGCAATCATCTTTAGCTTCTGAGGGGATCAATCACGATNT
GAACAGAGTAAAGAGTATACATTCATCTGATGGCCAATCTATAGAAAAGCGGCTTCCTGA
GGACTCAGATGTTGCCATGGAGGATGCTTGTTGCCATACTGAGAACCATGAACCAGAAAC
CGTGNATNATATGAGAACTGAGACAGAGACTGGCATCAGAGAAAATCAGATCAAGACACA
CTCG

Last Updated on 06.12.2018 16:11