Sequences from confirmation amplicon


Confirmation sequences for line SALK_046323c and hit At1g80760 are shown.
Line ID SALK_046323c
Locus At1g80760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30350901
Primers used for PCR 20ty - LBb3, show primer details
   
Sequence Name 32-K117180-SALK_046323C-20ty
GenBank Accession LN485173 [GenBank]
Sequencing primer gene specific primer, 20ty: 5'-AATGAATTAGAAGCAGCAATCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117180-SALK_046323C-20ty
TTTCTTAAAGATCTGTACGAGGCTAAAAACCAACCATTTTGATGAAACACTTGCAATTTT
GGTTAAAGGGCATTCCAAATTTTATAAGCATCGTCGACTTCAGATATTATATTATACACA
TATATTTATGTGATGTAAACAGAGTAATATAGAAGCGAGTGTTTTTCATCTTCTGAAGCT
CCTCCTCTCTTTGGGTGCTTCATCTTCCTCTGGCAACTTGACAATTGTGTATGTACCTGC
TCCGATTAACGCTCCAAGAATGGGGGCAGTGAGGTAAACCCAAATAGCTCTGTAATTGTT
TGCTGCAATGGCTGGACCCAGTGTTCTTACAGGGTTCATCGAAGCAGAAGTTGCAGGTCT
GTTGGACACAACATCATGATCAGTAACATGTATTATTATGTACAAGAACTGAATTCAAGA
AATGATGGTTTATTTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTT
   
Sequence Name 11-K117179-SALK_046323C-R210
GenBank Accession LN484595 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117179-SALK_046323C-R210
ATAACATCATTTCTTGATTCAGTTCTTGTACATAATAATACATGTTACTGATCATGATGT
TGTGTCCAACAGACCTGCAACTTCTGCTTCGATGAACCCTGTAAGAACACTGGGTCCAGC
CATTGCAGCAAACAATTACAGAGCTATTTGGGTTTACCTCACTGCCCCCATTCTTGGAGC
GTTAATCGGAGCAGGTACATACACAATTGTCAAGTTGCCAGAGGAAGATGAAGCACCCAA
AGAGAGGAGGAGCTTCAGAAGATGAAAAACACTCGCTTCTATATTACTCTGTTTACATCA
CATAAATATATGTGTATAATATAATATCTGAAGTCGACGATGCTTATAAAATTTGGAATG
CCCTTTAACCAAAATTGCAAGTGTTTCATCAAAATGGTTGGTTTTTAGCCTCGTACAGAT
CTTTTAAGGAAAATATAGTAAGTAGCTAGCGATTACAACGATTGCTGCTTCTAATTCATT
AAAAGATCTGTACGAGGCTAAAAACCAACCATTTTGATGAAACACTTGCAATTTTGGTTA
AAGGGCATTCCAAATTTTATAAGCATCGTCGACTTCAGATATTATAT

Last Updated on 06.12.2018 16:11