Sequences from confirmation amplicon


Confirmation sequences for line SALK_046603c and hit At3g56600 are shown.
Line ID SALK_046603c
Locus At3g56600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20969411
Primers used for PCR 39vi - LBb3, show primer details
   
Sequence Name 44-K117945-SALK_046603C-39vi
GenBank Accession LN485380 [GenBank]
Sequencing primer gene specific primer, 39vi: 5'-TTGTTTTGAATTTGCAGAAACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117945-SALK_046603C-39vi
AGGTCTCCCATTCGTGTTCCTAGGCTTACACACATGTTTTGATCAACAATTTCTTGTGTT
CATGAACTAGATATTGCAGGTAGTAATGGATTCAAAGAACACCATCTTATCTCCGAGTAT
CATCAATCCGCTTTGCTTGCTGTCCTGGAGGTGATTTGTCTTTTAGATGATTGACGAATT
CATCGAAGAAGAGTTGCCACCATTAATCTGATCAGCCAAAAAAAAGGTTTGGCATGAACT
GTATGGCCGTCGCTCTCGATCCACTAACCGACCGGTTTTCGCAGTTTAGCCGATCTTCAC
AGAGATGTAGACTCCAATCTTTAACCAACCTTGACTTCAACTTTCTCGGATTCAACACTA
AACAAACCAATCTCTCTGCTTCCTCACACTCCTTGAACAACCGAAGCGTTTCTACTCCAT
GCTTCTCCATATCCGGTAGTAATCTAGATGGCTCTGCTACTCCGCACATTGAGATTCTCG
GTGGTCAAAGAGTCCCCACTGTACGTTCCCTTGTAGCTGAAGTAACCATAGCTATCGTTT
CTGGAGCTCAGCCTCTGCTTGGTAACACATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 09-K117944-SALK_046603C-R210
GenBank Accession LN484534 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117944-SALK_046603C-R210
GCAGAGGCTGAGCTCCGAAACGATAGCTATGGTTACTTCAGCTACAAGGGAACGTACAGT
GGGGACTCTTTGACCACCGAGAATCTCAATGTGCGGAGTAGCAGAGCCATCTAGATTACT
ACCGGATATGGAGAAGCATGGAGTAGAAACGCTTCGGTTGTTCAAGGAGTGTGAGGAAGC
AGAGAGATTGGTTTGTTTAGTGTTGAATCCGAGAAAGTTGAAGTCAAGGTTGGTTAAAGA
TTGGAGTCTACATCTCTGTGAAGATCGGCTAAACTGCGAAAACCGGTCGGTTAGTGGATC
GAGAGCGACGGCCATACAGTTCATGCCAAACCTTTTTTTTGGCTGATCAGATTAATGGTG
GCAACTCTTCTTCGATGAATTCGTCAATCATCTAAAAGACAAATCACCTCCAGGACAGCA
AGCAAAGCGGATTGATGATACTCGGAGATAAGATGGTGTTCTTTGAATCCATTACTACCT
GCAATATCTAGTTCATGAACACAAGAAATTGTTGATCAAAACATGTGTGTAAGCCTATGG
AACACGAATGGGAGACCTTATAATCATTCACACTCCTGCAATTGTAAATAGCCGCAAGTT
TCTGCAATTTCAAAACAA

Last Updated on 06.12.2018 16:11