Sequences from confirmation amplicon


Confirmation sequences for line SALK_046841c and hit At3g18660 are shown.
Line ID SALK_046841c
Locus At3g18660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6421144
Primers used for PCR 25dh - LBb3, show primer details
   
Sequence Name 21-K109819-SALK_046841C-25dh
GenBank Accession LN484879 [GenBank]
Sequencing primer gene specific primer, 25dh: 5'-TAAGCAGAGAAGCAATATGCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K109819-SALK_046841C-25dh
ATCGGATATGGTGAAACCGTTTAATATCATAAATTTTTCGACACAAGACAAAAACAGTAG
TTGTTGTTGTTTCACCAAGTTTCAGATCGTGAAGCTTCTCTTGTTTATCCTTCTCTCTGC
CACTCTCTTCACCATTATCTATTCTCCTGAAGCTTATCATCATTCTCTTTCCCACTCATC
TTCTCGGTAAATCTATTTCTTTTTTCCATCACCAACATTTACATTCTTGACCTCAAAAAT
GTTCACATGCAAATTTTTACTTTTGCCTCTATCTCTTATAATACTATCTTAAAATTATGA
AATTAGATGGATATGGAGAAGACAAGATCCACGTTACTTCTCGGATCTGGATATAAACTG
GGACGATGTGACTAAAACCCTTGAGAACATCGAAGAAGGCCGTACGATCGGTGTCTTGAA
TTTTGATTCGAACGAGATCCAACGATGGAGAGAAGTATCCAAGAGCAAGGACAATGGGGA
TGAAGAAAAAGTTGTTGTATTGAATCTAGATTACGCAGACNAGAATGTGACTTGGGACGC
ACTATATCCAGAGTGGATCGATGAGGAGCAAGAAACAGAGGTCCCTGTTTGTCCTAATAT
TGTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCANGCGAAAATC
CTGTTTGATGGTGGTTC
   
Sequence Name 05-K109818-SALK_046841C-R210
GenBank Accession LN484395 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K109818-SALK_046841C-R210
ATTTGTTACACCACAATATTAGGACAACAGGGACCTCTGTTTCTTGCTCCTCATCGATCC
ACTCTGGATATAGTGCGTCCCAAGTCACATTCTTGTCTGCGTAATCTAGATTCAATACAA
CAACTTTTTCTTCATCCCCATTGTCCTTGCTCTTGGATACTTCTCTCCATCGTTGGATCT
CGTTCGAATCAAAATTCAAGACACCGATCGTACGGCCTTCTTCGATGTTCTCAAGGGTTT
TAGTCACATCGTCCCAGTTTATATCCAGATCCGAGAAGTAACGTGGATCTTGTCTTCTCC
ATATCCATCTAATTTCATAATTTTAAGATAGTATTATAAGAGATAGAGGCAAAAGTAAAA
ATTTGCATGTGAACATTTTTGAGGTCAAGAATGTAAATGTTGGTGATGGAAAAAAGAAAT
AGATTTACCGAGAAGATGAGTGGGAAAGAGAATGATGATAAGCTTCAGGAGAATAGATAA
TGGTGAAGAGAGTGGCAGAGAGAAGGATAAACAAGAGAAGCTTCACGATCTGAAACTTGG
TGAAACAACAACAACTACTGTTTTTGTCTTGTGTCGAAAAATTTATGATATTAAACGGTT
TCACCATATCCGATCTGCCACCTCCTTTGCTATTTCTCCGGAATCTCCTCTTGCATATTG
CTTCTCTG

Last Updated on 06.12.2018 16:11