Sequences from confirmation amplicon


Confirmation sequences for line SALK_047290c and hit At2g21040 are shown.
Line ID SALK_047290c
Locus At2g21040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9026580
Primers used for PCR BI66 - R204, show primer details
   
Sequence Name 53-K107203-SALK_047290C-BI66
GenBank Accession LN485573 [GenBank]
Sequencing primer gene specific primer, BI66: 5'-TATGCAAGTCAACTTCACCATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K107203-SALK_047290C-BI66
GTATCGATGACGTGGCACTCGATAACTCTATTAGAAAACATAAGAATTTGTGCAAAAAAA
CCTTCAATGTAATTTTGTTTTTGCAAGATAAACCTCCATGTCAAATAAATATAGCATAGG
TCAAACCACAATCTGAAAAATCTCCTACAACATAACTCTTCCGTGTATCGATGACGTGAC
AGTCGGTAACTCATTTAGAGATTTTATAAGTCTATCGAATATATTATTTTATTGCATATA
ATTTAATTGAGTTATTGATTTAAGTTTTAAATAAACTATAAGAGAAGCGTTAGGTCATCG
GGGGTTTCTCCTGATGTCATGATTCAATAAATAAGGTATAGATCTCAATCTAGGTGATAA
CATGGTTAGGTTTGTTTACTTCAAAATTCTCGGACATACGAAACTCTATCTGAAAGAAAT
GGTGCTTGACGAACATAAAGCATTCACTTTGGAACTACGTAAGACTTTGGACGGTGGTCA
AGAACGGCAGCTTGTAGGGGGAAGCTGGAAGTTGAACTCTTGTATAAGTCATTCACGAAG
GAAGAAATGCAAGCGGTTCAGAAAGCTCCAGAAGGCACACCGGCTGCTGGAGGAATGTTT
GTGGTAATAGTGCATTCGGCTGAGGATGTTGAAGGAAAGCACCATACCAATCCTTACGTG
CACATCTACTTCAAAGGGGAGGAGAGAAAAACAAAGCATGTAAAGAAGAACAAAGACCCA
AAGTGGAATGAGGAGTTCTCCTTTATGCTGGAGGAGCCTCCAATCCATGAGAAAATGCAC
GTGAAAGTGTTTAGCACCTCTTCCAGGATAGTGTTTGTACTGAGGTTGTAACAATAAGTA
ATTAAAGGGTTTTAGGGTAAACAATTTCTTCTTTTGAATACTCGAGAACTCGGTCGTGCT
TTTGACAGACAAAAGTTTATTTTATTTTATAGATGCGCCTCGGTAGAGGCTGCCTACGTA
CCCTTTG
   
Sequence Name 45-K107202-SALK_047290C-R210
GenBank Accession LN485385 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K107202-SALK_047290C-R210
GTCATTTGTTTACCCCAATATATCCTGTTAAGCGCCTTCAAAGGGTACGTAGGCAGCCTC
TACCGAGGCGCATCTATAAAATAAATAAACTTTTGTCTGTCAAAAGCACGACCGAGTTCT
CGAGTATTCAAAGGAAGAAATTGTTTACCCTAAAACCCTTTAATTACTTATTGTTACAAC
CTCAGTACAAACACTATCCTGGAAGAGGTGCTAAACACTTTCACGTGCATTTTCTCATGG
ATTGGAGGCTCCTCCAGCATAAAGGAGAACTCCTCATTCCACTTTGGGTCTTTGTTCTTC
TTTACATGCTTTGTTTTTCTCTCCTCCCCTTTGAAGTAGATGTGCACGTAAGGATTGGTA
TGGTGCTTTCCTTCAACATCCTCAGCCGAATGCACTATTACCACAAACATTCCTCCAGCA
GCCGGTGTGCCTTCTGGAGCTTTCTGAACCGCTTGCATTTCTTCCTTCGTGAATGACTTA
TACAAGAGTTCAACTTCCAGCTTCCCCCTACAAGCTGCCGTTCTTGACCACCGTCCAAAG
TCTTACGTAGTTCCAAAGTGAATGCTTTATGTTCGTCAAGCACCATTTCTTTCAGATAGA
GTTTCGTATGTCCGAGAATTTTGAAGTAAACAAACCTAACCATGTTATCACCTAGATTGA
GATCTATACCTTATTTATTGAATCATGACATCAGGAGAAACCCCCGATGACCTAACGCTT
CTCTTATAGTTTATTTAAAACTTAAATCAATAACTCAATTAAATTATATGCAATAAAATA
ATATATTCGATAGACTTATAAAATCTCTAAATGAGTTACCGACTGTCACGTCATCGATAC
ACGGAAGAGTTATGTTGTAGGAGATTTTTCAGATTGTGGTTTGACCTATGCTATATTTAT
TTGACATGGAGGTTTATCTTGCAAAAACAAAATTACATTGAAGGTTTTTTTGCACAAATT
CTTATGTTTTCTAATAGAGTTATCG
Primers used for PCR 72xo - R212, show primer details
   
Sequence Name 82-K121396-SALK_047290C-72xo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 72xo: 5'-CAAGTTTTCCAAGGAATAGTGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K121396-SALK_047290C-72xo
TACACATGAATATGCGCGTGATCTCGATCATATATTATCAATAGCAAGTAATCAACTTAC
AACTTACAATTATACAACTTGATCATGTGTATTTATTAACTTAATCGAGTTGAGATATTA
TATAAATTAATATAGAAGTTGACCAACTATTATTTTATTATTTTGTAATGATCGTGAAAC
TTGAAGCTAGAGAAAGTTGATTTAAGAAAAAATCTCAAATCAGAGGGTCGACCCTGAGAT
TTTAGAGGCCACACACCGACTCCTGACGAACCGGCTCCTTGACGAACCGGCTCCTTGACG
AACCGGCTCCCTGACAAACCAGCTCCCTGACGAACCGCCTCCTGACGAACCAGCTCCCTG
ACGAACCGCCTCCTGACGAACCAGCTCCCTGACGAACCAGCTCCCTGACGAACCAGCTTC
CTAACGAACCGACTCCTTGACGAACCGCCTCCTGACAAACCAAGCTTGTGACAATCTACA
CCGAATAAATGGTCTAGCCGAACCAAGCTCTCCATGCCATGTTAATCTCTTCGAAAGACT
CCCTCACCATTACGTGACAAGTCAAGACTCCCTCGTCGTGATGAGCCAACATTCTCACCA
TTACTAGCCAACGTCTTTTCCTCTAGCTAAACCATCCCGCACAACAAAGTCGTCTCGCAN
AGTATAATCATCAAGTAAAACATAGCCATCCCGCCNAGTAGAGCCATATCTCCAAACGAA
GTCGTATCGCCAAACCCTTTACTGCAAGCGGATCCATCGCTAAACAACTTAGCTCCTCGT
CTAACTGTCGACGTCATCACTTAAC
   
Sequence Name 93-K121474-SALK_047290C-R212
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K121474-SALK_047290C-R212
CGCGGACTGAGTGGCTCCTTCATCGTTGCGGTTCTGATCAGTTCGAACGTAAAACGGCTT
GTCCCGCGTCATCGGCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGA
TTGTGTTATTAAGTTGTACCCTTTCAAGCCGTTCGTAGTTCATGTTACATAGGTGAAAGC
TTGTAGTTTGGAACTATGCGCTTTGGAGCCAAGCAGTTAAGTGATGACGTCGACAGTTAG
ACGAGGAGCTAAGTTGTTTAGCGATGGATCCGCTTGCAGTAAAGGGTTTGGCGATACGAC
TTCGTTTGGAGATATGGCTCTACTTGGCGGGATGGCTATGTTTTACTTGATGATTATACT
TTGCGAGACGACTTTGTTGTGCGGGATGGTTTAGCTAGAGGAAAAGACGTTGGCTAGTAA
TGGTGAGAATGTTGGCTCATCACGACGAGGGAGTCTTGACTTGTCACGTAATGGTGAGGG
AGTCTTTCGAAGAGATTAACATGGCATGGAGAGCTTGGTTCGGCTAGACCATTTATTCGG
TGTAGATTGTCACAAGCTTGGTTTGTCAGGAGGCGGTTCGTCAAGGAGTCGGTTCGTTAG
GAAGCTGGTTCGTCAGGGAGCTGGTTCGTCAGGGAGCTGGTTCGTCAGGAGGCGGTTCGT
CAGGGAGCTGGTTCGTCAGGAGGCGGTTCGTCAGGGAGCTGGTTTGTCAGGGAGCCGGTT
CGTCAAGGAGCCGGTTCGTCAAGGACCCGTTCGTCAG

Last Updated on 06.12.2018 16:11